天津市儿童流行性感冒病原学检测分析

郭小华; 段卫平; 王世荣; 张华; 李颖; 陈锦英

Abstract

郭小华,段卫平,王世荣,张华,李颖,陈锦英.天津市儿童流行性感冒病原学检测分析[J].Chinese journal of Epidemiology,2003,24(1):19-21

天津市儿童流行性感冒病原学检测分析

Analysis on the aetiology of influenza in children in Tianjin

Received:August 27, 2002

DOI：

KeyWord: 流感病毒血凝试验血凝抑制试验

English Key Word: Influenza virus Hemag glutination test Hemagg lutination inhibition test

FundProject:

Author Name	Affiliation
Guo Xiao-hua	Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
Duan Wei-ping	Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
Wang Shi-rong	天津市第三中心医院, 天津 300011
Zhang Hua	天津儿童医院, 天津 300011
Li Ying	天津医科大学, 天津 300011
Chen Jin-ying	天津医科大学, 天津 300011

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Abstract:

目的: 对天津市2001～2002年流行性感冒(流感)患儿进行病原学分析。方法：用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。用鸡红细胞和人O型红细胞凝集试验证实病毒的存在, 用红细胞凝集抑制试验进行型和亚型的鉴定。结果: 2001年10月至2002年3月共采集14岁以下流感样患儿咽拭子标本238份, 分离出64株流感病毒, 检出率为26.9%; 其中A(H3N2 )亚型42株, 占检出数的65.6%; A(H1N1)亚型13株, 占20.3 %; B型9株, 占14.1%。所分离的 64株流感病毒都具有适应于MDCK细胞株生长及与人O型红细胞凝集良好的生物学特性。转种鸡胚后, 绝大多数(6264株)能适应鸡胚生长; 但64份阳性咽拭子标本液直接接种鸡胚, 仅有 3份血凝阳性。此外, 96.4%(5355株)A型流感病毒均可由O相转为D相, 有2株A(H3N2)为O相特征。 B型流感病毒均为D相特征。结论: 天津地区流感病毒存在A(H3N2)、A(H1A1)和B三个型, 以A(H3N2)为优势流行型。

English Abstract:

Objective： To analyse the pathogen of child patients with influenza in Tianjin aera. OMethods：The influenza virus isolation was performed by MDCK cells and embryonated eggs. The identification of the isolates was carried out with hemag glutination (HA)and hemag glutination inhibition (HI)tests. Results： Two hundred and thirty-eight throat swab specimens from children with influenz-like illness were collected in Tianjin aera from Oct. 2001 to Mar.2002 and 64 strains (26.9 %)of influenza viruse were isolated. Data showed that there were 42 strains (65.6%) of A(H3N2) subty pe, 13 strains(20.3%)of A(H1N1)subty pe and 9 strains (14.1%) of B type in these positive isolates. All the isolated viruses grew very well in MDCK cells and hemagglutinated with human “O” red blood cells, and most (6264 strains)of them were able to multiply in embryonated chick eggs. However, there were only 3 isolates with HA positive in inoculating embryonated eggs with the specimens. Meanwhile, it was revealed that out of 55 strains of A type viruses, 53 strians (96.4 %)were from O to D phase, 2 strain of A(H3N2)were D phase characters and all B type isolated viruses being D phase properties. Conclusion：There were three endemic types of influenza viruses-A(H3N2), A(H1N1)and B type in Tianjin aera, with A, the main type.

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