| 闫杰,何忠平,庄辉,董庆鸣,宋淑静,朱林,王小红.中国北方5城市慢性乙型肝炎患者中SEN病毒检测[J].Chinese journal of Epidemiology,2003,24(1):33-35 |
| 中国北方5城市慢性乙型肝炎患者中SEN病毒检测 |
| Detection of SEN virus in sera of patients with chronic hepatitis B and general population in 5 cities of China |
| Received:April 10, 2002 |
| DOI： |
| KeyWord: SEN病毒 乙型肝炎 慢性 巢式聚合酶链反应 |
| English Key Word: SEN virus Hepatitis B, chronic Nest-po lymerase chain reactions |
| FundProject:北京市卫生局肝炎重点学科资助 |
| Author Name | Affiliation | | YAN Jie | Beijing Ditan Hospital, Beijing 100011, China | | HE Zhong-ping | Beijing Ditan Hospital, Beijing 100011, China | | ZHUANG Hui | 北京大学医学部病原生物学系, 北京 100011 | | DONG Qing-ming | Beijing Ditan Hospital, Beijing 100011, China | | SONG Shu-jing | Beijing Ditan Hospital, Beijing 100011, China | | ZHU Lin | 北京市东城区疾病预防控制中心流行病科, 北京 100011 | | WANG Xiao-hong | 北京大学医学部病原生物学系, 北京 100011 |
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| Abstract: |
| 目的: 了解SEN病毒D和H(SENV DH)在中国慢性乙型肝炎患者(CHB)中的感染情况。方法: 应用巢式聚合酶链反应法 (nPCR), 对中国北方5城市595例CHB患者和北京市96名正常人血清检测SENV D HDNA, 并用PCR产物直接测序法对7株SENV进行了序列分析。 结果: CHB患者SENV感染率为61.3%, 正常人为62.5%, 两者差异无显著性(χ2 =0.047,P =0.829)。 但不同城市CHB患者中SENV感染率存在一定差异。 从不同城市分离的SENV的核苷酸序列同源性较高, 4株SENV D同源性为91%～98%, 3株SENV H同源性为95%～98%。结论：在5城市CHB患者中存在SENV DH感染, 其感染率与正常人群相似, 提示SENV DH可能没有直接致病性。 |
| English Abstract: |
| Objective: To study the prevalence of SEN virus (SENV)infection in CHB patients in five cities of China. Methods: A nest-polymerase chain reaction (nPCR) was used for detection of SENVD and SENV-H in sera of 595 CHB patients from 5 cities of China and 96 normal individuals from Beijing. A total of 7 SENV strains were analyzed by direct sequencing. Results: The prevalence rates of SENV in CHB patients and normal individuals were 61.3% and 62.5%, respectively (χ2 =0.047, P=0 .829). The prevalence rates of CHB patients between 5 cities were different. Nucleotide sequence analysis showed that the homology between 4 SENV-D strains was 91%-98% and 95%-98% between 3 SENV-H strains isolated from 5 cities in China. Conclusion: SENV-D H were prevalent in CHB patients of China and their prevalence rates were similar to that in normal individuals. |
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