广东省江门地区登革病毒分离株的鉴定及结构蛋白序列分析

任瑞文; 方美玉; 洪文燕; 黄宝明; 蒋廉华; 刘建伟; 田小东; 程刚锋

Abstract

任瑞文,方美玉,洪文燕,黄宝明,蒋廉华,刘建伟,田小东,程刚锋.广东省江门地区登革病毒分离株的鉴定及结构蛋白序列分析[J].Chinese journal of Epidemiology,2003,24(4):288-290

广东省江门地区登革病毒分离株的鉴定及结构蛋白序列分析

Guangdong province jiangmen region of dengue virus isolates identification and structural protein sequence analysis

Received:May 10, 2002

DOI：

KeyWord: 登革病毒病毒分离序列分析

English Key Word: Dengue virus Viral isolation Sequence analysis

FundProject:全军医学科研“十五”计划重大课题资助项目( 0 1Z0 14 )

Author Name	Affiliation
Ren Ruiwen	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Fang Meiyu	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Hong Wenyan	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Huang Baoming	Jiangmen city, guangdong province health epidemic prevention station
Jiang Lianhua	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Liu Jianwei	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Tian Xiaodong	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory
Cheng Gangfeng	510507, guangzhou military area commands LianQinBu military institute of medical microbiology laboratory

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Abstract:

目的对广东省江门地区 2 0 0 1年夏、秋季一批发热、出疹患者进行确诊,并从分子水平分析流行株的可能来源。方法分别采用免疫荧光、细胞毒力、乳鼠毒力实验以及逆转录聚合酶链反应 (RT PCR)技术进行病毒鉴定,并对其结构蛋白基因序列进行克隆、测序及同源性搜索。结果37份患者血清登革病毒 (DV)IgM抗体阳性率为 97% (36 37),IgG抗体阳性率为 59% (2 2 37),最高抗体滴度均可达 1∶640。所得病毒可致C6 36细胞病变,具有乳鼠神经毒力 ;其结构基因序列长度为2 32 5bp,编码 774个氨基酸 ;与其他登革 2型病毒株TSV0 1、GD0 6 93、NGC、44、ThNH、0 4、GD0 8 98及S1进行比较,其核酸序列同源性 (% )分别为 98、96、94、94、92、92、92、91。b>结论 2 0 0 1年江门地区登革热流行为登革 2型病毒感染所致,推测其可能是输入性传染

English Abstract:

Purpose Purpose for guangdong province jiangmen region in summer, autumn 2001 confirmed a group of patients with fever, rash, and from the potential sources of molecular level analysis of epidemic strains. Methods respectively using immunofluorescence, cell toxicity, rats toxicity experiment and reverse transcription-polymerase chain reaction (rt-pcr) technique to identify the virus, and its structural protein gene sequences of cloning, sequencing and homology search. Results The results of 37 patients with dengue virus (DV) serum IgM antibody positive rate was 97% (36/37), IgG antibody positive rate was 59% (22/37), top have antibody concentration of 1, 640. The virus can cause C6/36 cell pathological changes, have rats nerve toxicity;Its structure gene sequence length is 2 325 bp, encoding 774 amino acids;With other dengue type 2 virus strain TSV01, GD06/93 and NGC 98, 44, ThNH, 04, GD08 / S1 and comparison, the nucleic acid sequence homology (%) is respectively 98, 96, 94, 96, 94, 92, 92, 92. conclusion The conclusion jiangmen region 2001 dengue fever epidemic of dengue type 2 virus infection caused by, speculated that it may be imported.

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