| 张志凯,俞东征,张建华,海荣,蔡虹,魏建春.布氏田鼠鼠疫菌102 kb pgm基因座结构研究[J].Chinese journal of Epidemiology,2003,24(4):291-295 |
| 布氏田鼠鼠疫菌102 kb pgm基因座结构研究 |
| Yersinia pestis 102 KB PGM loci m. brandti structure research |
| Received:June 20, 2002 |
| DOI： |
| KeyWord: 鼠疫耶尔森氏菌 布氏田鼠 毒力岛 插入序列 |
| English Key Word: Yale's plague bacteria Brandt's voles Virulence island Insert the sequence |
| FundProject: |
| Author Name | Affiliation | | Zhang Zhikai | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory | | Yu Dongzheng | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory | | Zhang Jianhua | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory | | Hai Rong | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory | | Cai Hong | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory | | Wei Jianchun | 102206, Beijing, the Chinese center for disease control and prevention of infectious diseases prevention and control of plague laboratory |
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| Abstract: |
| 目的 研究布氏田鼠鼠疫菌株 1 0 2kbpgm基因座结构与其他类型鼠疫菌是否有区别,及其与布氏田鼠鼠疫菌株的独特特征的关系。方法 采用聚合酶链反应 (PCR)的方法,共设计 2 5对嵌套的引物,以喜玛拉雅旱獭鼠疫菌株和布氏田鼠鼠疫菌株的染色体DNA为模板,分段扩增该区域内的DNA,选择差异较大的扩增片段进行克隆和测序,与已发表的序列比较。结果 布氏田鼠鼠疫菌株的 1 0 2kbpgm基因座一端缺失了1 952个碱基,即插入序列IS1 0 0。另外,在测序的这段基因中,一类似于可变数量串联重复序列 (VNTR)的区域,布氏田鼠鼠疫菌比已发表的序列多出几个拷贝。b>结论 布氏田鼠鼠疫菌株的 1 0 2kbpgm基因座序列改变了,一端缺失了插入序列IS1 0 0,这就使得它的毒力岛不容易丢失,保持了 pgm+表现型的稳定 ;与其毒力的关系,有待于进一步研究 |
| English Abstract: |
| Purpose Objective to study the plague m. brandti strain 102 KB PGM loci structure and other types of yersinia pestis whether there's a difference, and its unique characteristics with plague strains of m. brandti. Methods using the method of polymerase chain reaction (PCR), a total of 25 pairs of nested design, in order to Himalayan marmot plague strains and plague strains of m. brandti chromosome DNA as a template, segmented amplification of DNA in the area, choose different amplification segments by cloning and sequencing, compared with the published sequence. Results The results of plague strains of m. brandti 102 KB of PGM loci end missing 1 952 bases, namely IS100 insertion sequences. In addition, in sequencing the genes, a similar to the variable number tandem repeats (VNTR) area, yersinia pestis m. brandti out a few more copies than the published sequences. Conclusions plague strains of m. brandti 102 KB PGM loci sequence is changed, one end is missing IS100 insertion sequences, which makes its virulence island is not easy to loss, maintain the stability of the PGM + phenotype;Instead of the relationship between virulence, subject to further study. |
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