| 俞东征,海荣,董兴齐,李敏,夏连续,史献明,魏建春,崔百忠,王鹏,孙连芝,张志凯,胡源,张恩民.中国鼠疫菌种资源遗传特征分析[J].Chinese journal of Epidemiology,2003,24(11):1005-1009 |
| 中国鼠疫菌种资源遗传特征分析 |
| GenetiCanalysis of Yersiniapestis strains isolated in China |
| Received:June 18, 2003 |
| DOI： |
| KeyWord: 鼠疫菌 资源 遗传特征 |
| English Key Word: Yersiniapestis Resource Genetic characteristic |
| FundProject: |
| Author Name | Affiliation | | YU Dong-zheng | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | HAI Rong | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | DONG Xing-qi | 云南省地方病防治研究所 | | LI Min | 青海省地方病防治研究所 | | XIA Lian-xu | 内蒙古地方病防治研究所 | | SHI Xian-ming | 河北省鼠疫防治研究所 | | WEI Jian-chun | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | CUI Bai-zhong | 青海省地方病防治研究所 | | WANGpeng | 云南省地方病防治研究所 | | SUN Lian-zhi | 内蒙古地方病防治研究所 | | ZHANG Zhi-kai | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | HU Y uan | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | ZHANG En-min | Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: |
| 目的建立《中国甲类传染病菌种资源库》,选择适当的指标,以反映中国鼠疫菌种的遗传背景。方法使用传统的和分子生物学方法,测定中国鼠疫菌种代表菌株的遗传特征。并对遗传特征的分布及其意义进行分析。结果利用6项指标,将中国的鼠疫菌划分成15种遗传型,每一型占据一片相对独立的而又自相延续的地理空间。分析遗传型之间的亲缘关系,并根据菌株间的差异,追溯鼠疫耶尔森菌作为一个生物种形成后的进化过程。结论中国各鼠疫自然疫源地存在着不同特征的鼠疫菌株,但为达到对菌株认同的目标,还需要确定更多的遗传指征 |
| English Abstract: |
| Objective The strainsof Yersiniapestis isolated in differentperiod and differenTnatural foci in China were analyzed. Methods Traditional and molecular biological Methods were used. Rhamnose fermentantion, rRNA gene copy number, nitrite reduction, and the glycerol fernentation were importanTchararcters for typing, and pulse field gel electrophoresis( PFGE)and random amplified polymorphic DNA ( RAPD)profile could reflecTthe genetic distance between the strains.Results The strains could be dividedinto 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.Conclusion The characters of strains were described;the genetic relationship of defferenTtypes, their evolution, and the forming and shifTof plague natural foci were analyzed. |
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