| 孙利炜,童志礼,李丽红,章菁,陈琦,郑丽舒,刘静,谢华萍,王承训,张丽杰,Ivanoff B,Glass RI,Bresee JS,Jiang XI,Kilgorepe,方肇寅.长春市儿童医院1998～2001年轮状病毒哨点监测分析[J].Chinese journal of Epidemiology,2003,24(11):1010-1012 |
| 长春市儿童医院1998～2001年轮状病毒哨点监测分析 |
| Surveillance finding on rotavirus in Changchun children's hospital during July 1998-June 2001 |
| Received:March 27, 2003 |
| DOI： |
| KeyWord: 轮状病毒 监测 腹泻 儿童 哨点医院 |
| English Key Word: Rotavirus Surveillance Diarrhea Children Sentinel hospital |
| FundProject:世界卫生组织基金(V27181123);美国NIH基金(R03TW01192);国家“863”基金资助项目(2001AA212171) |
| Author Name | Affiliation | E-mail | | SUN Li-wei | Changchun Childr en’s Hospital, Changchun 130061, China | | | T ONG Zhi-li | 中国疾病预防控制中心病毒病预防控制所, 130061 | | | LI Li-hong | Changchun Childr en’s Hospital, Changchun 130061, China | | | ZHANG Jing | 中国疾病预防控制中心病毒病预防控制所, 130061 | | | CHEN Qi | Changchun Childr en’s Hospital, Changchun 130061, China | | | ZHENG Li-shu | 中国疾病预防控制中心病毒病预防控制所, 130061 | | | LIU Jing | Changchun Childr en’s Hospital, Changchun 130061, China | | | XIE Hua-ping | 中国疾病预防控制中心病毒病预防控制所, 130061 | | | WANG Cheng-xun | Changchun Childr en’s Hospital, Changchun 130061, China | | | ZHANG Li-jie | 中国疾病预防控制中心病毒病预防控制所, 130061 | | | Ivanoff B | Department of Vaccines and Biologicals, WHO CDC USA | | | Glass RI | Viral Gastroenteritis Section OH USA | | | Bresee JS | Viral Gastroenteritis Section OH USA | | | Jiang XI | Children’s Hospital Medical Center, Cincinnati | | | Kilgorepe | International Vaccine Institute, Seoul Korea | | | FANG Zhao-yin | 中国疾病预防控制中心病毒病预防控制所, 130061 | fangzhyn@263.net |
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| Abstract: |
| 目的为在中国开发和应用轮状病毒疫苗提供流行病学背景资料。方法以医院为基础的哨点监测,在长春市儿童医院5岁以下腹泻患儿中进行,收集患儿临床资料和粪便标本,轮状病毒检测用聚丙烯酰胺凝胶电泳(PAGE)和或酶联免疫吸附试验(ELISA),毒株分型用ELISA和或逆转录聚合酶链反应(RTPCR)。所有资料录入计算机进行数据分析。结果3年监测中共调查2343例腹泻患儿,收集便样1211份,轮状病毒检出率门诊患儿和住院患儿分别为31.0%和52.9%。轮状病毒感染95.0%发生于2岁以下儿童。每年寒冷季节流行(11月到次年3月)。流行的轮状病毒G血清型依次为G1(82.4%)、G2(5.0%)、G3(3.3%)和G4(0.9%),P基因型以P[8]和P[4]为常见。共检出9种P～G结合的毒株,其中世界常见的4种流行株占75.6%。结论轮状病毒流行毒株呈现超常多样性,轮状病毒是长春地区儿童重症腹泻的主要原因 |
| English Abstract: |
| Objective To establish baseline patterns of rotavirus diarrhea and to describe its epidemiologic features in Changchun city, prior torotavirus vaccine immunization. Methods Hospita-l based surveillance was conducted among children under 5 years old with acute diarrhea in Changchun Children s Hospital. Fecal samples were determined to identify rotavirus by PAGE and or ELISA. G serotypes of rotavirus were identified by ELISA and or nested RT-PCR. P genotyping were carried ouTby RT-PCR. All data were computerized and analysed by Generic Manual on Rotavirus Surveillance seTby CDC in the USA. Results In total, 2 343 diarrhea cases were screened and 1211 fecal samples were collected. Rotavirus was detected in 31. 0% among outpatients and 52. 9% in inpatients. During thepeak of the season ( November through March), 58. 6% of diarrhea was caused by rotavirus among inpatients. 95. 0% of rotavirus diarrhea cases occurred among children aged < 2 years. The predominanTstrain was serotype G1 (82. 4%), followed by G2 (5. 0%), G3 ( 3. 3%), G4 ( 0. 9%). P genotyping showed thaTP [ 8] and P [4] were the mosTcommon ones. Nine differenTP-G combinations were identified, with four worldwidely commonly seen strains (P [8] G1, P [4] G2, P [ 8] G3, and P [8]G4), accounted for 75. 6% of the total, together with uncommon strains including the novel types P [4] G4 and P [8] G2, which highlightied the extraordinary diversity of rotaviruses circulating in China. Conclusion Rotavirus is the major cause of severe child diarrhea in Changchun. Developing a rotavirus vaccine for prevention of severe disease and reduction of treatmenTcosts seemed to be necessary. |
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