| 糜祖煌,秦玲.无锡地区淋病奈瑟菌TEM-1基因分子流行病学研究[J].Chinese journal of Epidemiology,2004,25(1):58-59 |
| 无锡地区淋病奈瑟菌TEM-1基因分子流行病学研究 |
| A molecular-epidemiologic study on TEM-1 genes of Neisseria in Wuxi area |
| Received:December 25, 2002 |
| DOI： |
| KeyWord: 淋病奈瑟菌 基因 流行病学 分子 |
| English Key Word: Neisseria gonorrhoeae Gene Epidemiology molecular |
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| Abstract: |
| 目的 调查无锡地区淋病奈瑟菌株TEM-1基因流行情况。方法 参照国外的β；-内酰胺酶阳性的淋病奈瑟菌菌株内含TEM-1基因的质粒序列，自行设计淋病奈瑟菌TEM-1基因的半套式聚合酶链反应（heminested PCR）检测方法。并对2002年1~；10月自无锡地区分离的195株菌进行检测。结果 195株淋病奈瑟菌临床分离株中有138株为TEM-1基因阳性，阳性率为70.8%；。1株无锡地区分离株的TEM-1基因半套式PCR产物，经DNA测序与核酸库登录的β；-内酰胺酶阳性的淋病奈瑟菌质粒pFA7序列99%；同源。结论 含TEM-1基因的淋病奈瑟菌株是无锡地区的流行株。 |
| English Abstract: |
| Objective In order to study the epidemiology of TEM-1 genes of Neisseria gonorrhoeae （Ng） in Wuxi area.Methods In the light from foreign literature that pooitive Ng strains of β-lactamase contain plasmid sequences of TEM-1 genes, heminested PCR for TME-1 genes of Ng detection was self-designed.Ng of 195 strains were detected, that were isolated in Wuxi area from Jan to the Oct, 2002.Results There were 138 TEM-1 genes positive in 195 isolated strains with a positive rate of 70.8%.There was one case of heminested PCR product of TEM-1 genes which showed the homogeneity was 99%in Wuxi area, when comparing with pFA7 sequences of positive Ng plasmid of β-lactalase from register GenBank.Conclusion Data showed that the Ng strains with TEM-1 genes were the prevalent ones in Wuxi area. |
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