| 汤芳,刘玮,张泮河,赵秋敏,詹琳,左曙青,吴晓明,杨红,方立群,魏茂提,王宏伟,曹务春.SARS患者粪便、痰标本病毒RNA阳性检出率与病程变化的关系[J].Chinese journal of Epidemiology,2004,25(2):120-123 |
| SARS患者粪便、痰标本病毒RNA阳性检出率与病程变化的关系 |
| Study on the correlation between positive rates of SARS RNA in clinical confirmed SARS patients and the appearance of RNA in relation to the development of the disease |
| Received:September 29, 2003 |
| DOI： |
| KeyWord: 严重急性呼吸综合征 冠状病毒 逆转录-聚合酶链反应 |
| English Key Word: Sever acute respiratory syndrome SARS associated coronavirus Reverse transcription-polymerase chain reaction |
| FundProject:国家重点发展计划“863”计划资助项目(2003AA208406) |
| Author Name | Affiliation | | TANG Fang | Institute of Microbiology and Epidemiology, Beijing 100071, China | | LIU Wei | Institute of Microbiology and Epidemiology, Beijing 100071, China | | ZHANG Pan-he | Institute of Microbiology and Epidemiology, Beijing 100071, China | | ZHAO Qu-min | Institute of Microbiology and Epidemiology, Beijing 100071, China | | ZHAN Lin | Institute of Microbiology and Epidemiology, Beijing 100071, China | | ZUO Shu-qing | Institute of Microbiology and Epidemiology, Beijing 100071, China | | WU Xiao-ming | Institute of Microbiology and Epidemiology, Beijing 100071, China | | YANG Hong | Institute of Microbiology and Epidemiology, Beijing 100071, China | | FANG Li-qun | Institute of Microbiology and Epidemiology, Beijing 100071, China | | WEI Mao-ti | Institute of Microbiology and Epidemiology, Beijing 100071, China | | WANG Hong-wei | Institute of Microbiology and Epidemiology, Beijing 100071, China | | CAO Wu-chun | Institute of Microbiology and Epidemiology, Beijing 100071, China |
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| Abstract: |
| 目的 探讨严重急性呼吸综合征(SARS)患者粪便、痰标本中病毒RNA阳性检出率与病程变化的关系。方法 采取一步法逆转录聚合酶链反应(RT-PCR)对不同病程的临床标本进行SARS冠状病毒(SARS-associated coronavirus,SARS-CoV)RNA的扩增和序列测定,并采用趋势X~2检验探讨标本阳性检出率与不同发病时间的关系。结果 被检样品中扩增出的特异性片段,经测序验证为SARS-CoV序列(同源性100％)。在检测的临床标本中,粪便标本阳性检出率最高(21.55％)。趋势X~2检验显示SARS患者粪便和痰标本中病毒阳性检出率均随着发病时间的增长而下降,X~2=12 55和16.408,P=0.0004和P=0.000 05。结论 一步法RT-PCR可以有效检测SARS患者临床标本存在的SARS-CoV;SARS患者粪便、痰标本RT-PCR阳性率随着发病时间的增长而下降。 |
| English Abstract: |
| Objectives To study the correlation between positive rates of RNA in clinical confirmed severe acute respiratory syndrome (SARS) patients and its appearance in relation to the development of the disease in order to provide scientific basis for early diagnosis, effective prevention and treatment of the disease. Methods One-step reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the SARS RNA in the clinical specimens from different courses of the disease. The representative amplicons were then sequenced. Chi-square for trend test was performed to study the correlation between positive rates of RT-PCR and at different periods after the onset of the disease. Results The fragments amplified from the sputum specimens of SARS patients were shown to share 100% homology with the published SARS- associated coronavirus. Of the different clinical specimens, positive rate in the stools appeared to be the highest (21. 55%). Chi-square for trend test revealed that the positive rates of stools and sputa of SARS patients decreased with the development of the disease (X~2 for trend = 12. 55 and 16. 408, P = 0. 0004 and P = 0. 000 05 respectively). Conclusion One-step RT-PCR proved to be an effective method for the detection of SARS-associated coronavirus from clinical specimens. Data as indicated that the positive rates o SARS coronavirus were decreasing in SARS patients along with the disease progession. |
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