| 张利军,熊鸿燕,张耀,马非,李莉.营养启动剂诱导类炭疽芽胞发芽的研究[J].Chinese journal of Epidemiology,2005,26(3):207-210 |
| 营养启动剂诱导类炭疽芽胞发芽的研究 |
| Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant |
| Received:September 16, 2004 |
| DOI： |
| KeyWord: 类炭疽芽胞 发芽 营养启动剂 |
| English Key Word: Bacillus anthr acoides,spore Germination Nutrient germinant |
| FundProject:全军“十五”科技基金资助项目(01MA170) |
| Author Name | Affiliation | E-mail | | ZHANG Li-jun | Department of Military Epidemiology,Academy of Preventive Medicine,The Third Military Medical University, Chongqing 400038, China | | | XIONG Hong-yan | Department of Military Epidemiology,Academy of Preventive Medicine,The Third Military Medical University, Chongqing 400038, China | hyxiong@mail.tmmu.com.cn | | ZHANG Yao | Department of Military Epidemiology,Academy of Preventive Medicine,The Third Military Medical University, Chongqing 400038, China | | | MA Fei | Department of Military Epidemiology,Academy of Preventive Medicine,The Third Military Medical University, Chongqing 400038, China | | | LI Li | Department of Military Epidemiology,Academy of Preventive Medicine,The Third Military Medical University, Chongqing 400038, China | |
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| Abstract: |
| 目的 观察营养启动剂诱导类炭疽芽胞杆菌芽胞发芽的生物效应。方法 通过热激活和营养启动剂诱导类炭疽芽胞发芽分光光度计测量芽胞悬液在600 nm的吸光度值(A),计算不同条件下的发芽率透射电镜观察发芽过程中芽胞超微结构的变化。结果 在 37℃ pH 7.9的条件下,6 mmol/L次黄嘌呤核苷营养诱导剂使类炭疽芽胞发芽率为68.0%在 30℃ pH 8.9的条件下,70 mmol/L L-丙氨酸营养诱导剂使类炭疽芽胞发芽率为74.5%两者的混合营养诱导剂可明显提高发芽率,达到85.6%透射显微电镜下观察,见类炭疽芽胞出现外衣及皮质层破裂、降解,核心暴露。结论 在人工调整的适宜环境下,以次黄嘌呤核苷和L-丙氨酸为主要成分的营养启动剂可快速启动类炭疽芽胞发芽。 |
| English Abstract: |
| ObjectiveTo explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant.MethodsHeat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the waveleng th of 600 nm. Accrding to the A value, the g ermination r ates in different condition. Transmission electron microscope was used to obser ve the ultrastructur e chang es of spores.ResultsThe rate of germination effects were 68.0 % under 6 mmol/L inosine at 37, pH 7. 9;74.5% under 70 mmol/ L L-alanine at 30, pH 8.9;and 85.6% under 6 mmol/ L inosine and 70 mmol/ L L-alanine at 37, pH 8.2.Under transmission electron microscope, the germinated spores coat and cortex were brokendown and degraded with its core completely exposed.ConclusionUnder suitable environment,the nutrient germinant with inosine and L-alanine might be helpful for germinat ing the bacillus anthracoides spores. |
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