| 孙黎,张永振,李林红,张彦平,张爱梅,郝宗宇,孙建伟,陈化新.河南省Ⅱ型汉坦病毒基因亚型及其分布的研究[J].Chinese journal of Epidemiology,2005,26(8):578-582 |
| 河南省Ⅱ型汉坦病毒基因亚型及其分布的研究 |
| Genetic subtypes and distribution of Seoul virus in Henan |
| Received:November 11, 2004 |
| DOI： |
| KeyWord: 汉坦病毒 亚型 分布 |
| English Key Word: Hantavirus Genetic subtype Distribution |
FundProject:科技部科研院所社会公益研究专项资金重点项目(2001DIA40037,2002DIB40095);
国家“十五”科技攻关课题资助项目(2003BA712A0802) |
| Author Name | Affiliation | | SUN Li | College ofLife Science, Xinjiang Shihezi University, Shihezi 832003, China Corresponding author: | | ZHANG Yong-zhen | ZHANG Yong-zhen, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | LI Lin-hong | 河南省疾病预防控制中心 | | ZHANG Yan-ping | 河南省疾病预防控制中心 | | ZHANG Ai-mei | 河南省疾病预防控制中心 | | HAO Zong-yu | 河南省疾病预防控制中心 | | SUN Jian-wei | 河南省疾病预防控制中心 | | CHEN Hua-xin | College ofLife Science, Xinjiang Shihezi University, Shihezi 832003, China Corresponding author: |
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| Abstract: |
| 目的查明河南省Ⅱ型汉坦病毒基因亚型的分布情况。方法在河南省汉坦病毒流行严重的地区,捕捉啮齿类动物,以免疫荧光法检测阳性标本,应用汉坦病毒型特异性引物以逆转录聚合酶链反应方法对抗原检测阳性的鼠肺标本扩增M和S基因片段上的特异核苷酸序列并测序,将扩增片段的核苷酸序列与已知病毒序列进行比较,以明确基因亚型及其地理分布情况。结果褐家鼠、黄胸鼠以及小家鼠携带的病毒均为Ⅱ型汉坦病毒。序列分析发现河南省主要疫区宿主动物间流行的Ⅱ型汉坦病毒至少存在S1、S2和S3三个亚型,其中S1和S3为河南省汉坦病毒的优势流行基因亚型。结论河南省汉坦病毒的亚型较为复杂,地理分布较广。 |
| English Abstract: |
| Objective To investigate the sub-genotypes and distribution of Seoul virus in Henan. Methods Rodents were collected in the major epidemic areas and rats lungs were studied by indirect immunofluorescence assay. Partial M and S segments were amplified with nested reverse transcription-polymerase chain reaction using Hantavirus genotype-specific primers, sequenced,analyzed and compared with other known sequences. Results The Hantavirus carried by Rattus norvegicus, Rattus flavipectus and Mus musculus were all belonged to Seoul virus in the main epidemic areas of Henan. We constructed two phylogenetic tree based on the partial M and S segment sequences while phylogenetic analysis distinguished three genetic subtypes (S1,S2 and S3). S1 and S3 were found main subtypes in Henan. Conclusion The results indicated that the genetic subtypes of Hantavirus were complicated and widely distributed in Henan. |
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