| 吴万敏,王晓娟,朱慧芬,沈关心.用噬菌体肽库筛选SARS病原体的模拟抗原表位[J].Chinese journal of Epidemiology,2005,26(11):904-906 |
| 用噬菌体肽库筛选SARS病原体的模拟抗原表位 |
| Study on the mimic epitopes screening program severe acute respiratory syndromes antigen with random phage peptide library |
| Received:November 19, 2004 |
| DOI： |
| KeyWord: 严重急性呼吸综合征 噬菌体肽库 模拟抗原表位 |
| English Key Word: Severe acute respiratory syndromes Phage peptide library Mimic antigen epitope |
| FundProject:内蒙古自治区卫生厅自然科学基金资助项目(20035028) |
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| Abstract: |
| 目的 筛选SARS病原体的模拟抗原表位. 方法 以混合SARS患者恢复期血清Ig作为筛选分子, 从噬菌体肽库中筛选出能与患者血清Ig结合的模拟抗原表位, 并研究其抗原特性. 结果 得到2个能与SARS患者恢复期血清Ig特异性结合的阳性克隆;DNA测序结果显示这2个模拟抗原表位与SARS抗原无同源性. 结论 用噬菌体肽库成功筛选得到SARS的模拟表位, 为开展用SARS模拟抗原表位探索SARS的防治研究创造了条件. |
| English Abstract: |
| Objective To screen the severe acute respiratory syndromes (SARS) mimotopes with random phage peptide library and to investigate their immunogenicity. Methods Using SARS sera as selective molecule, a 12 mer phage peptide library was biopanned and positive clones containing the mimic epitopes were selected. The immuno-characteriation of the epitopes were then investigated. Results 2 positive clones that having specific affinity to SARS sera were obtained. The DNA sequencing data showed no homology between the sequences of the deduced amino acid of the two mimic antigen peptides and the sequence of SARS. Conclusion SARS mimotopes were obtained by phage peptide library screening. This method might provide a new approach for SARS therapy and vaccine development. |
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