| 叶新华,金玉,方肇寅,孙亚萍,谢华萍,章青,DuncanSteele,RogerGlass.兰州地区2004-2005年度婴幼儿病毒性腹泻的病原学研究[J].Chinese journal of Epidemiology,2006,27(2):117-122 |
| 兰州地区2004-2005年度婴幼儿病毒性腹泻的病原学研究 |
| Etiological study on viral diarrhea among children in Lanzhou, Gansu, fr om July 2004 thr ough June 2005 |
| Received:November 17, 2005 |
| DOI： |
| KeyWord: 轮状病毒 病毒性腹泻 婴幼儿 |
| English Key Word: Rotavirus Viral diarrhea Children |
| FundProject:国家自然科学基金资助项目(30270069);世界卫生组织基金资助项目(V27/181/123);美国PATH基金资助项目(GAV.1142-01-07228-LPS);甘肃省科技攻关资助项目(2GS054-A43-014-27) |
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| Abstract: |
| 目的了解兰州地区主要四种腹泻病毒的流行病学特点。方法收集兰州大学第一医院儿科2004年7月至2005年6月5岁以下全部住院腹泻患儿400例的粪便标本,分别采用Dako公司酶免疫试剂盒检测轮状病毒、星状病毒、腺病毒,杯状病毒检测采用酶免疫法和逆转录-聚合酶链反应(RT-PCR)法。对轮状病毒、星状病毒阳性标本用RT-PCR进行毒株分型鉴定。结果400份标本中四种病毒检测阳性率依次为轮状病毒47.3%、杯状病毒15.5%、星状病毒9.5%、腺病毒7.5%。其中有混合感染的病例数占13.5%。轮状病毒毒株G血清型分型结果为G2(34.4%)、G3(32.8%)、G1(1.1%)、不同型混合感染(5.8%)、未能分型(25.9%),P基因型分型结果为P[4](45%)、P[8](22.1%)、未能分型(32.9%)。G型与P型组合P[4]G2(43.6%)、P[8]G3(25.6%),P[4]G3(13.8%)、P[8]G2(3.2%)、P[4]G1和P[8]G1各1例。星状病毒血清分型结果为1型(57.8%)、3型(2.6%)、8型(2.6%)、未能分型(36.8%)。病毒性腹泻的高发季节轮状病毒最为明显为10-12月份。发病年龄主要为2岁以下婴幼儿,轮状病毒的高发年龄是6-23月龄。结论兰州地区婴幼儿病毒性腹泻的病原复杂,轮状病毒仍是最主要病原,该年度轮状病毒的主要流行株为P[4]G2,与往年明显不同,病原混合感染比例较大,值得重视。 |
| English Abstract: |
| Objective To study molecular epidemiology of four major etiological viruses among children wit h acute diarrhea in Lanzhou, Gansu province. Methods Stool specimens wer e collected from all 400 inpatients less than 5 years old with acute diar rhea admitted in Department of Pediatrics, the First Hospital, Lanzhou University from Jul. 2004 through Jun. 2005. Dako IDEIATM kits were used for detection of rotavir us, adenovirus and astrovirus. Calicivirus detection was done by both reverse transcription-polymerase chain reactions (RT2PCR) and ELISA. Further strain characterization of rotavirus and astrovirus was carried out with RT2PCR. Results A tot al of 400 stool samples were collected and tested. Rotavirus was present in 47. 3% of the cases: calicivirus in 15. 5%, astrovirus in 9. 5% and adenoviruses in 7. 5%. Mixed infections with mult iple enteric viruses were present in 13. 5% of all samples. Among 189 rotavirus positive samples, serotype G2(34. 4%) was the predominant str ain followed by G3 (32. 8%), G1 (1. 1%) and mixed2G infection (5. 8%). 25. 9% of strains remained to be non2 typeable. Pgenotyping showed P[4] (45%) was most common followed by P[ 8] (22. 1%), and non2 typeable(32. 9%). Strain P[4] G2 (43. 6%) was the most common combination followed by P[8] G3(25. 6%), P[4]G3(13. 8%), and P[8]G2, P[ 4] G1 and P[8] G1. Among 38 astrovirus strains, serotypes1(57. 8%) was the predominant. Serotype 3 and 8 were found only in one case r espectively, 14 strains r emained to be non2t ypeable. A peak admission of rotavirus diar rhea was observed from October t hrough December. More than 95. 0% of viral diarrhea patients under hospitalization occurred among children younger than 2 years. The incidence rates of rotavirus were highest in infants aged 6223 months. Conclusion Rotavirus was the most important pathogen for viral diarrhea among children hospitalized in Lanzhou followed by calicivirus, astrovir us, and adenovirus. The predominant rotavirus strain cir culatedwas P[4] G2, which was different from the finding in the previous years. T he high rate of mixed infection with different viral agents was notable. |
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