| 李伟,崔志刚,张政,田克诚,钟豪杰,朱凤才,梁未丽,章丽娟,阚飙.中国四省甲型副伤寒分离株脉冲场凝胶电泳分析及分型数据库建立[J].Chinese journal of Epidemiology,2006,27(10):871-874 |
| 中国四省甲型副伤寒分离株脉冲场凝胶电泳分析及分型数据库建立 |
| Molecular typing of Salmonella paratyphi A isolates from four provinces with pulse-field gel electrophoresis |
| Received:January 26, 2006 |
| DOI： |
| KeyWord: 甲型副伤寒沙门菌 脉冲场凝胶电泳 分子分型 |
| English Key Word: Salmonella paratyphi A Pulse-field gel elect rophoresis Molecular typing |
| FundProject:国家“十五”科技攻关课题资助项目(2003BA712A05-04) |
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| Abstract: |
| 目的对中国4个甲型副伤寒流行省份的分离株进行标准化脉冲场凝胶电泳(PFGE)分型分析。方法对来自4个省的甲型副伤寒沙门菌利用XbaⅠ酶切染色体DNA进行PFGE分析,利用BioNumerics建立数据库和进行相似性聚类。结果分离自1998-2002年的89株甲型副伤寒分离株共有11种PFGE带型,这些菌株中具有优势带型。有3种带型相似性在96．3%,这3种带型的菌株占到分析菌株的86．5%,并在不同省份和年度中出现。结论建立了中国甲型副伤寒沙门菌的用于网络监测分析的PFGE技术和数据库,近年中国部分省份的甲型副伤寒沙门菌分离株PFGE型别集中,存在同型菌株的广泛流行。 |
| English Abstract: |
| Objective To analyze the molecular types of Salmonella paratyphi A strains isolated in the recent years, and to construct the standard S. paratyphi A databank in the laboratory surveillance network PulseNet China. Methods S. paratyphi A isolates from 4 provinces were analyzed with the standard pulsed-field gel electrophoresis (PFGE) protocol used in PulseNet and their patterns compared. The databank was constructed with BioNumerics. Results Eleven PFGE patterns were obtained, in which 3 predominant patterns were identifies with a similarity coefficient of 96.3 % . The strains of these patterns, accounted for 86.5% of the analyzed strains, appeared in different provinces and years. Conclusion The databank of S. paratyphi A was constructed and could be used in laboratory surveillance of S . paratyphi A in PulseNet China. From the analyses on molecular typing of the isolates, data suggested that the predominant strains might cause the epidemics in different regions. |
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