| 陈颖丹,王聚君,周长海,许隆祺.混合血清检测在寄生虫病血清流行病学调查中的应用[J].Chinese journal of Epidemiology,2006,27(11):947-949 |
| 混合血清检测在寄生虫病血清流行病学调查中的应用 |
| Study on the mixed testing of serum samples in seroepidemioiogical survey of parasitic diseases |
| Received:June 02, 2006 |
| DOI： |
| KeyWord: 血清流行病学 混合血清检测 成本-效果分析 |
| English Key Word: Seroepidemiological survey Mixed testing of serum samples Hygienic economic costeffectiveness analysis |
| FundProject:卫生部“十五”科技重大资助项目(卫疾控发[2001]178号) |
| Author Name | Affiliation | | CHEN Ying-dan | Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai 200025, China | | WANG Ju-jun | Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai 200025, China | | ZHOU Chang-hai | Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai 200025, China | | XU Long-qi | Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai 200025, China |
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| Abstract: |
| 目的通过旋毛虫病、弓形虫病血清学检测,探讨了混合血清检测(混检)在寄生虫病血清流行病学调查中的应用及对成本-效果的影响。方法根据二项分布原理,探讨混合血清检测的可行性。旋毛虫病或弓形虫病血清学检测,采用3、5、10份等三种混检方法。同时对血清样品逐一检测和混合检测进行了成本．效果评价。结果只要混有1份弱阳性血清,旋毛虫病或弓形虫病的三种混检都呈阳性结果。如果混合的血清全部是阴性,旋毛虫病3、5、10份阴性血清样品混合各检测24组,全部呈阴性；弓形虫病3、5份阴性血清样品混合,检测12组,都呈阴性,10份阴性血清样品混合,检测18组中16组呈阴性,2组呈阳性。旋毛虫病或弓形虫病混检显示,混检效率与待检寄生虫血清学阳性率有关,血清学阳性率在10%时,4份混检效率较高,血清学阳性率在1%时,以10份混检效率较高,而当血清阳性率在0．1%时,增加混检样品数可明显减少检测次数,但前提条件是要保证混检样品中只要有1份阳性样品,混检时都能测出阳性,而且若全部是阴性样品混检,也不出现阳性。结论运用卫生经济学成本-效果分析,表明寄生虫病血清流行病学调查,混检成本低,尤其是对预期血清学阳性率较低(≤1%)的调查,混检可节省大量成本。 |
| English Abstract: |
| Objective In order to accumulate experiences for improving the efficiency in serological tests,the present study on mixed testing of serum samples was performed by taking the serological test of trichinellosis and toxoplasmosis as the examples,and had proved the effects on cost-effectiveness of seroepidemiological survey of parasitic disease with method of mixed-samples test.Methods According to the binomial distribution principle,to develop an approach to the feasibility of mixed testing of serum samples,and to work on a cost-effectiveness analysis of one-by-one testing and mixed testing using hygienic economic analysis method was performed.For serological test of trichinellosis and toxoplasmosis,3 kinds of mixed testing methods,namely 3 serum sample mixture,5 serum sample mixture and 10 serum sample mixture,were performed.Results The results showed that all the 3 kinds of mixed tests of trichinellosis and toxoplasmosis showing positive result if only 1 weak positive serum sample were mixed with.When the serum samples being mixed were all negative ones,then among the 24 groups tested with each kind of negative serum sample mixture of trichinellosis (3 serum samples,5 serum samples and 10 serum samples), they all showed negative.However,among the 12 groups tested with 2 kinds of negative serum mixture of toxoplasmosis (3 serum samples and 5 serum samples),all showed negative while among the 18 groups tested with the 10 serum sample mixture,16 groups showed negative and 2 were positive.The mixed testing of trichinellosis and toxoplasmosis showed that the efficiency of mixed testing was related to the serological positive rate of the parasitic diseases to be examined.When serological positive rate was 10%, the efficiency of mixed testing was higher in 4 serum sample group.When serological positive rate was 1%, the efficiency of mixed testing was higher in 10 serum sample group and when serological positive rate was 0.1%,the in crease of the size of mixed serum samples could decrease the number of testing,but the prerequisite was that there must be one positive sample,so that the positivity for all the mixed tests could be detected.If mixed testing were performed on all negative samples,no positivity could be detected. Conclusion The result of cost-effectiveness analysis demonstrated that for seroepidemiological survey of parasitic diseases,the cost for mixed testing was low,especially when the serological positive rate was expected low(≤1%),thus the mixed testing could save a large amount of the cost. |
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