| 韩晓旭,代娣,张岩,张旻,张子宁,刁莹莹,耿文清,尚红.中国部分地区HIV患者B′和B′/C亚型毒株tat第一外显子基因变异与HIV疾病进展关系的研究[J].Chinese journal of Epidemiology,2006,27(11):968-972 |
| 中国部分地区HIV患者B′和B′/C亚型毒株tat第一外显子基因变异与HIV疾病进展关系的研究 |
| Study on the relationship between the polymorphisms and secondary structure of tat exon-1 gene and HIV/AIDS progress in subtype B′and B′/C |
| Received:May 30, 2006 |
| DOI： |
| KeyWord: 人类免疫缺陷病毒1型 tat基因 变异 二级结构 |
| English Key Word: Human immunodeficiency virus tat gene Mutation Secondary structure |
| FundProject:国家“十五”科技攻关课题资助项目(2004BA719A12);辽宁省优秀青年科研人才培养资金资助项目(3040011);教育部博士点专项课题资助项目(20040159005) |
| Author Name | Affiliation | E-mail | | HAN Xiao-xu | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | DAI Di | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | ZHANG Yan | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | ZHANG Min | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | ZHANG Zi-ning | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | DIAO Ying-ying | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | GENG Wen-qing | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | SHANG Hong | Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China | p3lab@yeah.net |
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| Abstract: |
| 目的了解中国B′和B′/C亚型HIV/AIDS患者tat第一外显子的基因序列及其二级结构的特征和变异特点,探讨其与HIV-1感染疾病进展之间的关系。方法从辽宁、吉林和云南省HIV-1感染者中选取病情呈缓慢进展的B′亚型感染者8例和B′/C亚型感染者5例,选择年龄、性别感染时间与前二者匹配的病情呈典型进展的B′亚型感染者26例和B′/C亚型感染者9例。采集外周静脉血,提取前病毒DNA,用巢式聚合酶链反应扩增HIV-1的tat基因,纯化后直接进行基因序列测定,序列编辑后翻译成氨基酸序列,进行氨基酸变异情况分析和二级结构预测。结果B′和B′/C亚型缓慢进展者、典型进展者Tat第一外显子中发现多种氨基酸替换,但除A58T外均未显示出与病毒载量以及疾病进展的明确相关性。23N、31S、32Y、46F变异均显示出亚型特异性；Tat蛋白的二级结构未发现规律性变化。结论中国HIV/AIDS患者tat第一外显子某些位点的基因变异,如A58T可能与病毒载量以及疾病进展有关,Tat蛋白的二级结构可能与HIV感染后的疾病进展无明显关系。 |
| English Abstract: |
| Objective To study the polymorphisms and secondary structure of human immunodeficiency virus (HIV-1) tat exon 1 among subtype B′and B′/C HIV-1 infected people in China and to explore the relationship between the polymorphism of tat exon 1 and the disease progression. Methods 8 subtype B′and 5 B′/C HIV-1 infected patients with slow disease progression were selected from Liaoning,Jilin and Yunnan province.26 subtype B′and 9 B′/C HIV-1 infected patients with similar sex,age but with typical disease progression were selected. Provirus was extracted from the whole blood. The gene sequences of the Tat exon 1 were amplified by nest-polymerase chain reaction (nest-PCR). Products were purified and sequenced directly.The sequences were aligned,translated,amino acid substitution were analyzed and secondary structures were predicted.Results Many amino acid substitution could be found in the exon 1 of Tat in HIV-1 subtype B′and B′/C recombinant strain infected persons with different disease progression except A58T,none of them showed definitely relationship with HIV viral load and disease progression.23N,31S,32Y and 46F were subtype-specific substitutions.No characteristic secondary structure of exon 1 of Tat was found.Conclusion Some of the mutations of tat exon 1 might be related to HIV viral load and disease progression.However,there was no relationship found between the secondary structure of Tat protein and the disease progression. |
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