| 李淑华,倪政,居丽雯,申惠国,谈逸云,蒋露芳,周连娣,林玉尊,郑英杰,姜庆五.上海地区2005年麻疹流行株基因特性分析[J].Chinese journal of Epidemiology,2007,28(2):165-168 |
| 上海地区2005年麻疹流行株基因特性分析 |
| Sequence analysis on measles viruses isolated in Shanghai in 2005 |
| Received:June 02, 2006 |
| DOI： |
| KeyWord: 麻疹病毒 基因型 序列分析 |
| English Key Word: Measles virus Genotype Seqence analysis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Li Shu-hua | School of Public Health, Fudan University, Shanghai 200032, China | | | Ni Zheng | 上海市闵行区疾病预防控制中心, 上海 200032 | | | Ju Li-wen | School of Public Health, Fudan University, Shanghai 200032, China | | | Shen Hui-guo | 上海市闵行区疾病预防控制中心, 上海 200032 | | | Tan Yi-yun | 上海市闵行区疾病预防控制中心, 上海 200032 | | | Jiang Lu-fang | School of Public Health, Fudan University, Shanghai 200032, China | | | Zhou Lian-di | School of Public Health, Fudan University, Shanghai 200032, China | | | Lin Yu-zun | School of Public Health, Fudan University, Shanghai 200032, China | | | Zheng Ying-jie | School of Public Health, Fudan University, Shanghai 200032, China | | | Jiang Qing-wu | School of Public Health, Fudan University, Shanghai 200032, China | jiangqw@shmu.edu.cn |
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| Abstract: |
| 目的: 了解2005年上海部分地区麻疹暴发流行株的基因型别和特性, 加强麻疹病毒学监测。方法: 采集暴发麻疹患者咽拭子标本分离病毒, 通过RT—PCR扩增麻疹病毒N基因C末端450bp核苷酸并进行序列测定, 与GenBank中麻疹病毒各基因型参考株及国内其他地区的麻疹分离株进行基因比较。结果: 从10例麻疹患者的咽拭子标本中共分离麻疹病毒4株, 均属H基因组H1基因型, 型内变异0.7%～1.3%。与HI型代表株China93—7的基因同源性为98%～98.2%; 与H2基因型代表株China94-I的基因差异为6.4%～6.9%。与A型代表株Edmonston的基因差异为6.7%～6.9%; 与中国麻疹疫苗株Shanghail91的基因差异为7.6%～8.0%, 与国内其他地区麻疹流行株基因差异为0.2%～3.7%。结论: 2005年上海部分地区麻疹暴发是由HI基因型病毒引起, 为中国本土流行株。 |
| English Abstract: |
| Objective: To ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005. Methods: Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein(N)gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucteotide with other measles virus strain published in GenBank. Resnlts 4 measles viruses were isolated from 10 throat swab specimens and the sequence analysis indicated that they belonged to H1 genotype. The homogeneity of 450 nucleotides in the C terminal of the N gene was at 98%-98.2% as compared to HI genotype(China93-7). They differed from genotype H2 (China94-1) at 6.4%-6.9% and from genotype A(Edrnonston)at 6.7%-6.9%, from measles vaccine (Shanghail91) at 7.6%-8.0%. They differed from the other measles viral strain isolated in China in 1993 2005 at 0.2%-3.7%. The variation within 4 isolated measles viruses was at 0.7%-1.3%. Conclusion: It was H1 genotype measles viruses, which are the native viruses in China that 1ed to the outbreak of measles in Shanghai, in 2005. |
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