| 丁静娟,刘悦晖,王梅.贵州省乙型肝炎病毒前C区及基本核心启动子变异分布[J].Chinese journal of Epidemiology,2007,28(2):169-172 |
| 贵州省乙型肝炎病毒前C区及基本核心启动子变异分布 |
| Study on the distribution of hepatitis B virus precore and basic core promoter mutations in Guizhou area |
| Received:July 26, 2006 |
| DOI： |
| KeyWord: 乙型肝炎病毒 变异 限制性片段长度多态性 |
| English Key Word: Hepatitis B virus Genotype Restriction fragment length polymorphism |
| FundProject:国家自然科学基金资助项目(30360098);贵州省优秀科技教育人才省长基金资助项目 |
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| Abstract: |
| 目的: 调查贵州省乙型肝炎病毒(HBV)前c区A1896、基本核心启动子区(BCP)T1762/A1764变异分布。方法: 收集贵阳、遵义、凯里、都匀4地区不同民族无症状携带者(Asc)、慢性肝炎(cH)、肝炎肝硬化(Lc)、肝细胞肝癌(HCC)患者血清482份, 用测序及限制性片段长度多态性检测A1896、T1762/A1764变异, 用s基因PCR—RFLP确定基因型。结果: A1896、T1762/A1764变异检出率分别为23.03%和29.67%。汉族感染者A1896、T1762/A1764变异检出率为27.64%和36.04%, 高于侗、苗、布依族感染者合并后的7.96%、8.85%(P<0.01)。A1896变异在B、C基因型中的分布为20.34%和27.13%(P>0.05), T1762/A1764变异为18.97%和46.28%(P<0.01)。A1896、T1762/A1764变异在HBeAg阴、阳性组问的分布差异有统计学意义(P值均<0.01)。从ASC到HCC组, A1896、T1762/A1764变异分布逐渐增高, LC、HCC组的检出率明显高于CH和ASC组(P值均<0.01)。A1896、T1762/A1764变异的分布: 贵阳(分别为31.79%和41.06%)高于遵义(10.94%和14.06%)、都匀(8.64%和11.11%)及凯里(2.86%和2.86%), 但多因素logistic回归分析在控制了HBeAg、HBV基因型及临床类型影响后, 在地区间分布差异无统计学意义。结论: A1896、T1762/A1764变异在贵州省不同民族问分布有-定差异。c型感染者易发T1762/A1764变异, 两种变异均与疾病进展有关。 |
| English Abstract: |
| Objective: To investigate the distribution of hepatitis B virus (HBV) precore A1896 and basic core promoter (BCP) T1762/A1764 mutations in Guizhou area. Methods: 482 patients with chronic HBV infection, belonging to 4 nationalities, including 225 asymptomatie carriers(ASC), 158 chronic hepatitis(CH), 57 liver cirrhosis(LC), 42 hepatocellular carcinoma(HCC), from 4 areas of Guizhou province were examined. HBV A1896 and T1762/A1764 mutations were determined by direct sequencing and restriction fragment length polymorphism(RFLP). HBV genotypes were determined by PCR-RFLP based on S gene. The relationship among these mutations and genotype and the progression of liver disease were studied by multi-normal logistic regression analysis. Results: A1896 and T1762/A1764 mutations were detected 23.03% and 29.67% among 482 patients. These mutations were more prevalent in Hans than in Dong, Miao and Buyi minorities (P<0.01, respectively). The mutations of A1896 and T1762/A1764 were inore commonly seen in HUeAg negative than in HgeAg positive patients (P<0.01, respectively). The mutation of T1762/A1764 was significantly higher in genotype C than in genotype B (P<0.01). There were significantly statistical differences in the detective rate of A1896 and T1762/A1764 mutations between patients with HCC. LC and CH.ASC. The distribution of these mutations in Guiyang (31.79% and 41.06%) was higher than in Zunye (10.94%, 14.06%)、Duyun (8.64%, 11.11%) or Kaili (2.86%, 2.86%). However, there was no statistical difference by multi-nonnal logistic regression analysis after controlling the influence of HBeAg statu, genotype and clinical types. Conclusion: The distributions of A1896 and T1762/A1764 mutations were different in some nationalities of Guizhou province. The mutation of T1762/A1764 was more commonly seen in genotype C than in genotypr B. These mutations were closely related to progression of ehronic liver diseases. |
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