乙型肝炎病毒基因型和前C及基本核心启动子突变与乙型肝炎疫苗阻断母婴传播的关系

王佳; 李杰; 庄辉; 刘社兰; 李荣成; 李艳萍; 梁争论

Abstract

王佳,李杰,庄辉,刘社兰,李荣成,李艳萍,梁争论.乙型肝炎病毒基因型和前C及基本核心启动子突变与乙型肝炎疫苗阻断母婴传播的关系[J].Chinese journal of Epidemiology,2007,28(4):331-333

乙型肝炎病毒基因型和前C及基本核心启动子突变与乙型肝炎疫苗阻断母婴传播的关系

Interruption failure of heptitis B virus vaccination in mother-to-infant transmission and heptitis B virus genotypes and preC/BCP mutations

Received:February 01, 2007

DOI：

KeyWord: 乙型肝炎病毒乙型肝炎疫苗基因型突变

English Key Word: Hepatitis B virus Hcpatitis B vaccine Genotype Mutation

FundProject:困家“l‘五”科技攻关课题资助项口(2004BA718802)

Author Name	Affiliation	E-mail
WANG Jia	Department of Microbiology, peking University Health Science Center Beijing 100083, China
LI Jie	Department of Microbiology, peking University Health Science Center Beijing 100083, China	jieli69@263 net
ZHUANG Hui	Department of Microbiology, peking University Health Science Center Beijing 100083, China	zhuangbmu@126．com
LIU Shelan	江苏省疾病预防控制中心
LI Rongcheng	广西壮族自治区疾病预防控制中心
LI Yanping	广西壮族自治区疾病预防控制中心
LIANG Zhenglun	中国生物制品检定所

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Abstract:

目的<\b> 探讨乙型肝炎(乙肝)病毒(HBV)基因型和前C及基本核心启动子(BCP)突变与乙肝疫苗阻断母婴传播的关系.方法<\b> 采集江苏省16对乙肝疫苗阻断失败的母婴血清样本32份,以及88对阻断成功的母婴血清样本176份.以型特异性引物PCR法检测16对乙肝疫苗阻断失败的母婴和88例阻断成功的母亲血清样本中HBV基因型,用PCR产物直接测序法检测HBV前C/BCP突变,采用Clustal W 1.8软件进行序列分析.结果<\b> 在16例阻断失败的母亲中,15例(93.8%)为HBeAg阳性,且均为C型(15/15,100%);88例阻断成功的母亲中,51例(58.0%)为HBeAg阳性,其中C基因型占45.1%(23/51).在HBeAg阳性母亲中,阻断失败组的C基因型检出率明显高于阻断成功组(χ2=14.3,P=0.003).但在C基因型HBeAg阳性母亲中,阻断成功组与失败组的T1762/A1764突变率差异无统计学意义(分别为13.3%和33.3%,P=0.4),且均无A1896突变.结论<\b> 感染HBV基因C型的母亲可能更易导致乙肝疫苗阻断失败,而前C/BCP基因突变与阻断母婴传播无关.

English Abstract:

Objective<\b> To investigate the association of heptitis B virus(HBV)genotypes and precore(PreC)／basal core promoter(BCP)mutation with interruption failure of HBV vaccination in motherto- infant transmission.Methods<\b> A total number of 208 serum samples were collected from infants and mothers，including 16 infants who had become HBsAg-positive despite a complete and timely course of immunization and another 88 infants successfully protected from mothe卜to infant HBV transmission.HBV genotypes were determined by type-specific primers PcR method.PreC／BcP mutations were detected by direct sequencing of PCR products，and Clustal W 1.8 software was applied to analyzing the sequences.Results<\b> 0f 16 mothers who were having vaccine failure infants，15(93.8％)were HBeAg positive and infected with genotype C(15／15，100％).Among 88 mothers of having children being protected by vaccine,51(58.0％)were HBeAg positive，with 45.1％(23／51)of genotype C The proportion of genotype C in HBeAg mothers of infants with vaccine failure，was significantly higher than that of mothers with vaccine protected infants(x^{2<\sup>=14.3，P=O.003).H。weVer，the frequencies of T1762／A1764
mutations had no significant differences between gcnotypc C HBeAg positive mothers with vaccine failure or protected infants(33.3％and 13.3％，respectively，P二O.4).N。A1896 mutation was found in these two growups.Conclusion<\b> HBV genotype C might contribute to the immune failure of HBV vaccination in mother_to-infant transmission，while PreC／BCP mutation might n。t have correlation with it.}

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