海荣,俞东征,史献明,张忠兵,唐永娇,王鹏,夏连续,魏绍振,徐兵,秦迎旭,张志凯,石国祥,徐冬蕾,蔡虹,张恩民,魏建春,耿英芝,黄德惠,赵斌,汪立茂,马凤琴,黄富,王月,张涛,张建华.基因和抗原检测技术在鼠疫监测中的应用与评价[J].Chinese journal of Epidemiology,2007,28(5):426-429
基因和抗原检测技术在鼠疫监测中的应用与评价
Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program
Objective To apply and eValuate new Methods regarding specific gene and antigen detection in plague survemance program．Methods 1798 samples from natural foci of plague were tested，using internal quality contml multiple—polymerase chain reaction，F1 antigen marked by jmmuno chromatographic assay and enzyme linked immunoSorbent assay．Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic Methods．ResuIts The overalI positive rate of culture on year“llio postis together with gene and antigen detection was 7．34％，showing an 16．8 1％increase when compa“ngto 6．28％ using y8"culture method alone．Therate of coincidence was 97．13％．Conclusion The new standard being used fof specific gene and antigen detection coukl increase the positive rate of diagnosis on plague.