| 杨萍,李强,赵志军.青海省贵德县2004-2006年大骨节病监测[J].Chinese journal of Epidemiology,2007,28(7):691 |
| 青海省贵德县2004-2006年大骨节病监测 |
| The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome |
| Received:March 15, 2007 Revised:June 08, 2012 |
| DOI： |
| KeyWord: 严重急性呼吸综合征 免疫荧光抗体试验 抗体 |
| English Key Word: Severe acute respiratory syndrome Immuno-fluorescence assay Antibody |
| FundProject:国家重点发展计划“863”计划资助项目(2003AA208406) |
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| Abstract: |
| 青海省贵德县东沟乡(新建坪、斜马浪、高红崖)3村是大骨节病历史重病区.1982年定为大骨节病病区,同年人群投服亚硒酸钠、维生素C、维生素E、硫代硫酸钠等药物,进行连续4年的综合防治,于1984年改水,1995年纳入国家监测点范围.现将该监测点2004-2006年的监测情况报告如下. |
| English Abstract: |
| Objective To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.Methods Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.Results The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM.Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (χ2 for trend=16.376, P=0.000 05 for IgG; χ2 for trend=28.736, P=0.000 00 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. Conclusions IFA can be used to assist diagnosis of SARS after 10 days of the onset of desease. |
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