| 何冬梅,邓峰,王洪敏,柯昌文,严纪文,朱海明,赖蔚苳,宋曼丹,杨冰,王
海燕,王建,马聪,柯碧霞,邓小玲,倪汉忠.广东省食品中单核细胞增多性李斯特菌脉冲场凝胶电泳技术分子分型的研究[J].Chinese journal of Epidemiology,2008,29(1):38-43 |
| 广东省食品中单核细胞增多性李斯特菌脉冲场凝胶电泳技术分子分型的研究 |
| Molecular typing of Listeria monocytogenes isolated from foodstuff in Guangdong province by pulsed-field gel electrophoresis |
| Received:April 12, 2007 Revised:June 01, 2012 |
| DOI： |
| KeyWord: 单核细胞增多性李斯特菌 脉冲场凝胶电泳技术 食品 分子分型 |
| English Key Word: Norovirus Acute viral gastroenteritis Epidemiology |
| FundProject: |
| Author Name | Affiliation | | HE Dong-mei | 中山大学公共卫生学院 | | DENG Feng | 广东省疾病预防控制中心微生物检验所,广州,510300 | | WANG Hong-min | 广东省疾病预防控制中心微生物检验所,广州,510300 | | KE Chang-wen | 广东省疾病预防控制中心微生物检验所,广州,510300 | | YAN Ji-wen | 广东省疾病预防控制中心微生物检验所,广州,510300 | | ZHU Hai-ming | 广东省疾病预防控制中心微生物检验所,广州,510300 | | LAI Wei-dong | 广东省疾病预防控制中心微生物检验所,广州,510300 | | SONG Mandan | 广东省疾病预防控制中心微生物检验所,广州,510300 | | YANG Bing | 广东省疾病预防控制中心微生物检验所,广州,510300 | | WANG Hai-yan | 广东省疾病预防控制中心微生物检验所,广州,510300 | | WANG Jian | 广东省疾病预防控制中心微生物检验所,广州,510300 | | MA Cong | 广东省疾病预防控制中心微生物检验所,广州,510300 | | KE Bi-xia | 广东省疾病预防控制中心微生物检验所,广州,510300 | | DENG Xiaoling | 广东省疾病预防控制中心微生物检验所,广州,510300 | | NI Han-zhong | 广东省疾病预防控制中心微生物检验所,广州,510300 |
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| Abstract: |
| 目的 应用脉冲场凝胶电泳技术(PFGE)对广东省食品中分离的单核细胞增多性李斯特菌(Lm)进行分子分型，并建立PFGE数据库.方法 参照美国PulseNet的Lm PFGE分子分型标准方法进行检测.应用BioNumerics软件对不同食物种类、时间和地点的分离株进行比对，分析菌株之间的相关性.结果 107株Lm分成41个PFGE型，型别较为分散|从鸡肉中分离的菌株数最多(37株)，PFGE分型也最多(19个)|冷藏和冷冻食品的分离率较高，其中有26株菌仅存在1～2个电泳条带的差异，相似度极高|韶关和惠州地区分离的Lm存在着地区特异性|随时间变迁，部分菌株仍存在不同程度的相关性.结论 广东省食品中分离的Lm在整体上表现出较大的遗传多样性，但部分菌株有不同程度的相关性。 |
| English Abstract: |
| Objective To investigate the epidemiological characteristics of norovirus infection in Beijing.Methods Both outbreaks and sporadic cases of acute gastroenteritis were studied through questionnaires while the stools of patients were collected.Noroviruses were detected by ELISA or RT-PCR, and PCR products were cloned and sequenced.Results Eight outbreaks were reported between November 2006 and March 2007,which were confirmed as nosocomial infections.A total of 158 positive cases were detected among 409 sporadic cases of acute virus gastroenteritis with a positive rate of 38.63%.The highest positive rate (55.00%) was found in group aged from 40 to 44,while the lowest positive rate (21.74%) fell into groups aged from 55 to 59.The positive cases aged from 6 months to 91 years with the mean age of 40 years old including 84 males and 74 females.Data from sequence analysis showed that norovirus epidemic strains belonged to the GⅡ/4 variants in Beijing,which were almost identical to the variants causing epidemics both in the Netherlands and in Japan.Conclusion Norovirus was important, causing virus-borne diarrhea between 2006 and 2007 in Beijing,and the epidemic strains were consistent with those isolated from both the Netherlands and Japan in 2006. |
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