| 王杰,高俊薇,庄辉,李杰,王佳,李雅娟,金晖.中国4城市慢性乙型肝炎病毒感染者中B基因亚型分布[J].Chinese journal of Epidemiology,2008,29(1):52-54 |
| 中国4城市慢性乙型肝炎病毒感染者中B基因亚型分布 |
| Study on the distribution of sub-genotype B on hepatitis B virus in patients with chronic HBV infection from 4 cities of China |
| Received:September 06, 2007 Revised:June 26, 2012 |
| DOI： |
| KeyWord: 乙型肝炎病毒 基因型 聚合酶链反应-限制性片段长度多态性 |
| English Key Word: Human metapneumovirus Infants and young children Acute respiratory infections |
| FundProject:国家自然科学基金资助项目(30570080);北京市自然科学基金资助项目(052020);北京市科委新星计划资助项目(2004B34) |
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| Abstract: |
| 目的 了解北京、石家庄、温州和深圳4城市慢性乙型肝炎(乙肝)病毒(HBV)感染者中B基因亚型(Ba和Bj)的分布情况.方法 应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)，对北京(18例)、石家庄(22例)、温州(34例)、深圳(27例)市101例乙肝患者血清标本，经多对型特异性引物巢式PCR鉴定为B基因型慢性HBV感染者的血清标本进行B基因亚型分析，并对部分血清标本以PCR产物直接测序法确定其亚型，以验证PCR-RFLP的准确性.结果 经PCR-RFLP分析101例均为Ba亚型，未发现Bj亚型，其中30例经测序分析也证实为Ba亚型.结论 4城市的HBV/B主要为Ba亚型。 |
| English Abstract: |
| Objective To understand the prevalence of human metapneumovirus(hMPV) infection in infants and young children in Beijing,China.Methods Gene fragment from hMPV was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with the primer pair located on the membrane (M) encoding gene.RNAs were extracted by Trizol from 3330 specimens collected from outpatients and inpatients with acute respiratory infections and visited the affiliated Children's Hospital from July 2004 to June 2006.These samples had been tested for conventional respiratory viruses including RSV,influenza A and B,parainfluenzaⅠ,Ⅱ,Ⅲand adenovirus by indirect immunofluoresence assay as well as virus isolation before RT-PCR for hMPV.Results Out of the 3330 clinical samples,110(3.3%) were found positive to hMPV.The ratio of male to female among those positive to hMPV was 1.5:1.Fifty-one out of 110 positive (46.4 96,51/110) specimens on hMPV were from children under one year olds,13 (11.8 %,13/ 110) were from 1 to 2 year olds,37 (33.6%,37/110)were from 2 to 5 years of age,and 9 (8.2%,9/110) among children over 5 years of age.hMPV was associated with:pneumonia in 44.5% (49/110) of the cases;upper respiratory infections in 22.7% (25/110);hronchiolitis in 10.9% (12/110);and bronchitis in 7.3% (8/110).hMPV was detected almost in every month from July 2004 to June 2005 in sporadic cases whereas a peak was noticed in April 2006 during the period from July 2005 to June 2006.Among 110 positive samples,6 (5.5 %) were also positive for other viruses including 3 as Influenza B,2 as RSV and 1 as Parainfluenza 3,suggesting that these children were co-infected with hMPV and other respiratory viruses. The peak of hMPV prevalence did not overlap with that of RSV during this period.Conclusion Our findings suggested that hMPV had been one of the important agents causing acute respiratory infections especially severe lower respiratory infections like pneumonia and bronchiolitis in pediatric patients in Beijing. Infants and young children under two years of age seemed to be more susceptible to hMPV. |
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