| 姚能云,徐平.博尔纳病毒感染的研究进展[J].Chinese journal of Epidemiology,2008,29(2):195-197 |
| 博尔纳病毒感染的研究进展 |
| Progress on the study of Borna disease virus infection |
| Received:July 26, 2007 Revised:June 28, 2012 |
| DOI： |
| KeyWord: 博尔纳病毒 感染 |
| English Key Word: Borna diwasP virus: Infection |
| FundProject:贵州省科技基金及贵州省省长基金资助项目(2055) |
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| Abstract: |
| 博尔纳病(Borna disease,BD)最早见于19世纪的德国,是一种在战马中流行的伴有神经精神症状的脑膜脑炎,致死率较高,这种疾病首次在德国萨克森州博尔纳镇暴发,后以该镇名字来命名. |
| English Abstract: |
| objective To establish a sensitive, specific, simple and practicable method for identifying the two sub-genotypes (Ba and Bj) of genotype B isolates of hepatitis B virus (HBV) (HBV/ B).Methods The entire nucleotide sequences of HBV/B and HBV/C were obtained from GenBank, compared and analyzed with DNAStar software.The specific primers for HBV/B (HB) and Ba (BA),Bj (BJ)were designed respectively.HB as HBV/B specific primer (sense) and HBAS-4V(designed by Japanese scientists Sugauchi et al) as a universal outer primer (antisense) were used in the first-round PCR.In the second-round PCR, HB was also used as sense primer white BA and BJ as inner primers (antisense) and they were added into a single tube for PCR reaction.The two sub-genotypes of HBV/B were identified according to the length of the PCR products.A total of 71 HBV DNA-positive serum samples were selected randomly from our laboratory, including 56 HBV/B samples identified by type-specified PCR method and 15 HBV/C samples identified by direct sequencing in preS and S Region (preS/S).A11 the 71 samples were detected with this semi-nested PCR method.A plasmid containing full length genomic DNA of HBV/Bj, was presented by Professor Kenji Abe as positive control of场、Then, the first-round PCR products of 15 HBV/B were randomly selected and sequenced directly, and their sequences were compared phylogenetically with the above known Ba and Bj sequences using Blast and DNAStar softwares to confirm the results of semi-nested PCR.Results 56 HBV/B samples were all identified as HBV/Ba by our semi-nested PCR method.15 randomly selected PCR products were all sequenced as HBV/Ba.A11 of the 15 HBV/C samples were negative.Conclusion A simple, rapid, sensitive and specific method for identifying sub-genotypes Ba and Bj was established whith might be used for large-scale clinical and epidemiological studies. |
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