| 张海林,唐青,陶晓燕,王晓光,张云智,杨卫红,米竹青,王静林,章域震,冯云,郑维斌,鲁国勇,李胜国.云南省保山市人间狂犬病调查及病毒分子生物学特征分析[J].Chinese journal of Epidemiology,2008,29(9):899-904 |
| 云南省保山市人间狂犬病调查及病毒分子生物学特征分析 |
| Survey on human rabies cases and its viral molecular biological features in Baoshan city, Yunnan province |
| Received:April 15, 2008 |
| DOI： |
| KeyWord: 狂犬病病毒|基因|分子流行病学 |
| English Key Word: Rabies virus|Cene|Molecular epidemiology |
| FundProject:国家高技术研究发展计划"863"资助项目(SQ2006AA022112882) |
| Author Name | Affiliation | | ZHANG Hai-lin | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | TANG Qing | 中国疾病预防控制中心病毒病预防控制所 | | TAO Xiao-yan | 中国疾病预防控制中心病毒病预防控制所 | | WANG Xiao-guang | 中国疾病预防控制中心病毒病预防控制所 | | ZHANG Yun-zhi | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | YANG Wei-hong | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | MI Zhu-qing | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | WANG Jing-lin | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | ZHANG Yu-zhen | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | FENG Yun | Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China | | ZHENG Wei-bin | 云南省保山市疾病预防控制中心 | | LU Guo-yong | 保山市隆阳区疾病预防控制中心 | | LI Sheng-guo | 腾冲县疾病预防控制中心 |
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| Abstract: |
| 目的 了解云南省保山市发生2例狂犬病患者的流行病学特点,根据病原分子生物学特征分析可能的传染来源。方法 采用狂犬病个案调查表进行流行病学调查,采集狂犬病死者脑组织标本,用直接免疫荧光试验(DFA)检测狂犬病病毒抗原,用RT-PCR法检测狂犬病病毒核酸,测定狂犬病病毒P、M和N基因全序列并根据其同源性比较及系统进化树进行分子流行病学分析。结果2006年7月保山市隆阳区发生1例人间狂犬病,2007年4月腾冲县发生1例狂犬病.2例患者犬伤暴露程度均为Ⅲ度。2例死亡病例均采集到脑组织(编号为CYN0601H和CYN0701H),经DFA和RT-PCR两种实验室方法检测确诊为狂犬病病毒阳性.CYN0601H和CYN0701H与国内外狂犬病病毒的P、M和N基因核苷酸和推导氨基酸序列同源性分析显示,CYN0601H和CYN0701H与泰国分离株的同源性最高.系统进化分析显示,2株病毒标本亲缘关系很近,同属于狂犬病病毒基因1型,且与泰国病毒株有较近的亲缘关系。结论从病原学和病毒分子水平证实云南省保山市2例患者均为狂犬病,保山市狂犬病病毒流行株可能为境外传人,应加强云南省边境地区狂犬病防制工作. |
| English Abstract: |
| Objective To understand the epidemiological features of two rabies cases in Baoshan city year 2006 and 2007 and to analyze its source of infection.Methods Questionnaires were used to do the epidemiologieal survey on each of the rabies cases.Brain timue samples of rabies patients were collet to detect the rabies virus by direct immunofluoreseence assay(DFA)and RT-PCR assay.Homology and phylogenetic tree were analyzed.based on the whole nucleotide and deduced amino acid sequence of P,M and N gene of rabies virus followed by molecular epidemiological analysis.Results In July 2006,one human rabies case was identified in Longyang district,and another one in Tengchong county in Baoshan city in 2007.The degrees of exposure of these two patients was all at degreeⅢ.Two brain tissue samples among the dead patients(No.CYN0601H and CYN0701H)were confirmed positive by both DFA and RTPCR assay.The homology analysis of P,M and N gene sequences among CYN0601H,CYN0701H and other rabias strains isolated from other provinces and other counties.showed that the samples in Baoshan city shared the highest homology with the strains in Thailand.Phylogenetic analysis indicated that the two samples were very dose and all belonged to genetype 1 Lyssavirus,with the closest relationship between samples in Baoshan city and strains in Thailand.Conclusion It Was confirmed on the virus molecular level that the two patients in Baoshan city were both suffered from rabies.The prevalent strains in Baoshan city WaS probably imported from foreign country,suggesting that prevention and control measures on rabies virus in the boarder areas of Yunnan should be strengthened. |
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