| 朱水荣,王志刚,陈恩富,杨婷婷,张政,徐宝祥,金大智,何剑琴,王复甦.浙江省散发猪链球菌病临床分离株存在89K候选致病岛[J].Chinese journal of Epidemiology,2008,29(11):1110-1113 |
| 浙江省散发猪链球菌病临床分离株存在89K候选致病岛 |
| The emergence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis isolated from sporadic patients in Zhejiang province |
| Received:April 07, 2008 |
| DOI： |
| KeyWord: 猪链球菌血清2型 聚合酶链反应 89K片段 |
| English Key Word: Streptococcus suis serotype 2 Polymerase chain reaction 89K DNA fragment |
| FundProject: |
| Author Name | Affiliation | | ZHU Shui-rong | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | WANG Zhi-gang | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | CHEN En-fu | 浙江省疾病预防控制中心传染病防制所, 杭州 310051
| | YANG Ting-ting | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | ZHANG Zheng | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | XU Bao-xiang | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | JIN Da-zhi | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China | | HE Jian-qin | 浙江大学附属第一医院卫生部传染病重点实验室 | | WANG Fu-su | The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China |
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| Abstract: |
| 目的 对浙江省临床分离猪链球菌血清2型(SS2)菌株进行89K候选致病岛检测.方法 用多种特异性引物进行PCR扩增检测,同时对89K的3个扩增片段进行测序,并结合有关资料进行生物信息学和流行病学分析.结果 8株SS2菌株,均检出89K(1&2)、89K(3&4)、89K(5&6)候选致病岛特异片段和种特异性16S rDNA、cps2J、mrp毒力基因,和1998年江苏省暴发疫情的菌株结果一致.ZJ0501号SS2分离株89K候选致病岛的3个PCR扩增片段序列和1998年江苏省暴发疫情的菌株有99%以上的相似性,其中编码DNA重组蛋白的序列片段和病乳链球菌及无乳链球菌有较高的同源性.结论 近年来在浙江省临床分离Ss2菌株中,存在89K候选致病岛片段. |
| English Abstract: |
| Objective jective To identify the presence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis serotype 2 (SS2) strains isolated from patient in Zhejiang province. Methods Genes and DNA fragments were amplified by PCR, using specific primers, and three amplified fragments of the89K sequence were directly sequenced. The results were analyzed using software related to bioinformaties and epidemiology. Results 8 strains of SS2 all contained 89K sequence, cps2J and mrp virulent genes, and species-specific 16S rDNA. 3 amplified fragments of 89K candidate pathogenicity island of SS2 ZJ0501 were above 99% similar to SS2 strain identified from outbreaks in Jiangsu in 1998, and the gene fragment of coding DNA recombinant protein in the 89K sequence was highly homological with that of S. dysgalactiae and S. agalactiae. Conclusion In recent years SS2 strains isolaed from patients with clinical symptoms in Zhejiang province had been detected to have contained candidate pathogenicy 89K DNA fragment. |
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