云南省楚雄州汉坦病毒宿主动物及分子流行病学研究

周济华; 张海林; 王静林; 杨卫红; 米竹青; 张云智; 章域震; 宋先毅; 胡秋凌; 董应宽; 普文华; 胡海梅; 高丽芬; 袁庆虹; 亚红祥; 冯云

Abstract

周济华,张海林,王静林,杨卫红,米竹青,张云智,章域震,宋先毅,胡秋凌,董应宽,普文华,胡海梅,高丽芬,袁庆虹,亚红祥,冯云.云南省楚雄州汉坦病毒宿主动物及分子流行病学研究[J].Chinese journal of Epidemiology,2009,30(3):239-242

云南省楚雄州汉坦病毒宿主动物及分子流行病学研究

Survey on host animal and molecular epidemiology of hantavirus in Chuxiong prefecture, Yunnan province

Received:October 26, 2008

DOI：10.3760/cma.j.issn.0254-6450.2009.03.009

KeyWord: 汉坦病毒汉城型宿主动物分子流行病学

English Key Word: Hantavirus Seoul type Host animal Molecular epidemiology?

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Author Name	Affiliation	E-mail
ZHOU Ji-hua	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
ZHANG Hai-lin	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000	zhangHL715@163.com
WANG Jing-lin	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
YANG Wei-hong	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
MI Zhu-qing	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
ZHANG Yun-zhi	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
ZHANG Yu-zhen	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
SONG Xian-yi	云南省楚雄州疾病预防控制中心
HU Qiu-1ing	云南省楚雄州疾病预防控制中心
DONG Ying-kuan	楚雄州卫生局
PU Wen-hua	云南省楚雄州疾病预防控制中心
HU Hai-mei	云南省楚雄州疾病预防控制中心
GAO Li-fen	云南省楚雄州疾病预防控制中心
YUAN Qing-hong	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
YA Hong-xiang	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000
FENG Yun	云南省地方病防治所/云南省病毒立克次体研究中心, 大理 671000

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Abstract:

目的掌握汉坦病毒(HV)在云南省啮齿动物中的流行情况及其遗传特征. 方法采用笼夜法捕鼠, 鼠肺标本用直接免疫荧光法检测HV抗原, 用RT-PCR法检测HV核酸及分型, 对产物进行序列测定, 用PHYLIP软件包进行系统发生分析. 结果 2005-2006年在云南省楚雄州10个县(市)居民区共捕获鼠类4属8种1421只, 其中褐家鼠1056只, 构成比高达74. 31%, 其次为黄胸鼠(19. 70%). 检测鼠肺组织1029份, HV抗原阳性53份, 总带病毒率为5. 15%, 带病毒鼠种为褐家鼠(5. 08%, 38/748))、黄胸鼠(5. 97%, 12/201)、大足鼠(7. 69%, 2/26)和斯氏家鼠(6. 25%, 1/16). HV汉城型(SEO)核酸检测阳性21份(褐家鼠15份, 黄胸鼠4份, 大足鼠2份). 获得的12个病毒S片段核苷酸序列与GenBank中国SEO型病毒R22、L99和HLD65株同源性较高, 为87. 1%～99. 7%;与汉滩型(HTN)76-118株同源性仅为64. 4%～69. 1%. 系统进化分析显示, 楚雄州12个病毒S片段核苷酸序列与SEO型亲缘关系较近, 与HTN型及其他型HV相距较远, 证实这12份病毒标本均为SEO型病毒, 并可分S1和S3两个亚型. 结论首次证实楚雄州10个县(市)广泛存在以褐家鼠为主要传染源的HFRS家鼠型疫源地及SEO型病毒的流行. 分析发现SEO型不同亚型病毒的分布有其地域特点和独立性.

English Abstract:

Objective To determine the hosts of hantavirus(HV)and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome(HFRS). Methods Rodents were captured by a special trap within the residential area. The antigens of HV in lung tissues were detected by direct immuno-fluorescence assay(DFA). Nucleotide sequences of HV were amplified by RT-PCR with HV genotype-specific primer. The amplified genes were then sequenced. Phylogenetic tree were built on nucleotide sequence with Clusta1X 1. 83 software. Results 1421 rodents were captured and classified into 8 species of 4 Genera in the epidemic area within 10 counties of Chuxiong prefecture, Yunnan province, between 2005 and 2006. Out of the 1421 rodents, 1056(74. 31%)of them were Rattus norvegicas and 280(19. 70%)belonged to Rattus flavipectus. The antigens of HV were detected by DFA in lung tissues and the total positive rate of HV was 5. 15%(53/ 1029). After applying the sequencing nucleotide method to the 53 positive specimens, data showed that 21 specimens were positive and all of them belonged to Seoul type( 15 samples were from Rattus norvegicus, 4 samples Rattasflavipectas, 2 samples Rattus nitidas). The partial S segments from 12 specimens were sequenced which appeared homologic with R22, L99 and HLD65 from GenBank in relatively high level(87. 1%-99. 7%). When compared to 76-118 strain of Hantaan type, their homologic degree was only 64. 4%-69. 1%. Results from Phylogenetic analysis showed that 12 specimens belonged to Seoul type. As for their homology, they were significantly similar to Seoul type and could be tentatively divided into two subtypes S1 and S3. Conclusion It was confirmed that the Seoul type virus, as HFRS's pathogenetic agent mainly carried by rats, prevailed widely in Chuxiong prefecture. Owing to the local ecological environment, we also noticed the characteristics of different HV subtypes among Seoul type.

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