| 郑会民,何纳,周艺彪,赵根明,姜庆五.应用聚合酶链反应检测日本血吸虫基因组DNA[J].Chinese journal of Epidemiology,2009,30(6):647-649 |
| 应用聚合酶链反应检测日本血吸虫基因组DNA |
| Polymerase chain reaction assay to detect the genomic DNA of Schistosoma japonicum |
| Received:October 29, 2008 |
| DOI： |
| KeyWord: 日本血吸虫 聚合酶链反应 基因组DNA |
| English Key Word: Schistosoma japonicum Polymerase chain reaction Genomic DNA |
| FundProject:国家"863"计划(2006AA02Z402);上海市重点学科建设计划(B118) |
| Author Name | Affiliation | E-mail | | ZHENG Hui-min | Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China | | | HE Na | Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China | | | ZHOU Yi-biao | Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China | | | ZHAO Gen-ming | Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China | | | JIANG Qing-wu | Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China | jiangqw@fudan.edu.cn |
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| Abstract: |
| 中国是日本血吸虫病主要流行区[1],疫情形势严峻.准确评估钉螺感染和水体污染情况,对疫情防制意义重大.传统的压碎法、逸蚴法和哨鼠法灵敏性都较低[2],影响了血防工作的效率.有人尝试将PCR方法用于血吸虫病的诊断和流行监测[3-5],其中根据埃及和曼氏血吸虫中发现的串联重复序列建立的PCR方法都表现出极高的灵敏性[6-8],实用前景乐观[9].本研究的主要目的是揭示日本血吸虫中可能存在的该序列,优化PCR条件,评价其灵敏性.…… |
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