| 刘静,杨洋,宫菊丽,张子宁,张昊,王亚男,姜拥军,戚中田,潘启超,钟平,尚红.HIV-1/HCV合并感染者中HCV基因亚型流行情况调查[J].Chinese journal of Epidemiology,2009,30(7):663-667 |
| HIV-1/HCV合并感染者中HCV基因亚型流行情况调查 |
| The prevalence of hepatitis C virus(HCV)subtypes in Chinese HIV.1/HCV CO-infected individuals |
| Received:January 07, 2009 |
| DOI：10.3760/cma.j.issn.0254-6450.2009.07.003 |
| KeyWord: 丙型肝炎病毒 人免疫缺陷病毒 合并感染 基因亚型 |
| English Key Word: Hepatitis C virus HINl Co-infection Subtype |
| FundProject:国家科技重大专项课题(2008ZXl0001—001);卫生部艾滋病防治应用性研究项目（WA2006-02）;上海市医学重点学科建设项目(05Ⅲ029). |
| Author Name | Affiliation | E-mail | | Liu Jing | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | | | YANG Yang | 解放军第二军医大学微生物教研室 | | | GONG Juli | 上海市预防医学研究院艾滋病病毒免疫研究室 | | | ZHANG Zining | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | | | ZHANG Min | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | | | WANG Yanan | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | | | JIANG Yonun | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | | | QIZhongtian | 解放军第二军医大学微生物教研室 | | | PAN Qichao | 上海市预防医学研究院艾滋病病毒免疫研究室 | | | ZHONG Ping | 上海市预防医学研究院艾滋病病毒免疫研究室 | | | SHANG Hong | 110001,沈阳,中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室 | hongshang100@hotmail.com |
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| Abstract: |
| 目的 了解中国部分地区HIV-1和HCV合并感染人群中HCV基因亚型的流行、分布及其与HIV-I感染疾病进展的关系。方法 对186份获自河南、云南、新疆、吉林和辽宁省HIV-1/HCV合并感染人群标本(HCV病毒载量>1000 cop/m1), 用反转录巢式聚合酶链反应方法 扩增血浆HCV核心基因区并进行基因亚型分型, 同时检测HIV-1和HCV载量以及CD, +T细胞计数。结果 (1)HCV不同基阒亚型比例分别为la(1.7％)、lb(39.9％)、2a(17.9％)、3a(10.4％)、3b(15.6％)、6a(1.2％)、6n
(6.4％)和6型未鉴定亚型(7.5％)。HCV 2a和lb主要流行于河南省既往有偿献血浆人员巾;3a和3b亚型主要流行于新疆和云南静脉注射吸毒者(IDU)中;HCV6型主要流行于云南吸毒人员中。(2)lb亚型的HCVRNA水平显著高于非lb亚型, 但在HIV-I载量和CD, +T细胞数方面差异无统计学意义。2a亚型的HIV-1 RNA和HCV RNA水平显著低于非2a亚型。结论 HIV-I/HCV合并感染人群中HCV基因亚型的流行和分布与流行地区和感染途径有关。新的HCV6型亚型病毒株已经在合并感染
的IDU人群中流行。尚未发现HCV基因哑型与HIV感染疾病进展之间的关系。 |
| English Abstract: |
| Objective To beaer understand the prevalence and geographic distribution of genotypes/subtypes on HCV and the relationship between HCV genotypes/subtypes and HIV infection disease progression in the HIV-l/HCV CO.infected individuals living in high HIV-1 prevalent areas in China.Methods 1 86 plasma samples were collected from HIV-1 seropositive individuals infected through paid blood donors(PBD), injecting drug USerS(IDUs)or sexual contact, living in most severely affected provinces, Henan, Yunnan, Xinjiang, Jilin and Liaoning provinces.Samples witll HCV viral load>1 000 cop/ml were amplified by RT-nested PCR, sequenced and phylogenetically analyzed for genotyping/subtyping of HCV：HIV-1.HCV viral loads and CD, +T lymphocytcs were measured for all subjects.Results (1)HCV were identified as la(1.7%), lb(39.9%), 2a(17.9%), 3a(10.4%), 3b(15.6%), 6a(1.2%), 6n(6.4%), and a newly unclassified subtype(7.5%).HCV 2a and 1b subtypespredominated in PBD in Henan, 3a and 3b in IDUs in Xinjiang and Yunnan, and 6 genotype/subtypes in IDU in Yunnan.(2)There were no significant differences in CD4+T eell counts among the different HCV subtypes.(3)The viral lpad of HCV RNA in lb subtype was higher than at of non-lb subtype, however.no significant differences in HIV1 virallpads and CD4+T eell counts were found between lb and non.1b subtype.Both HIV and HCV viral loads were lower in 2a than non.2a subtype.Conclusion The prevalence of HCV genotype/subtype in HIV.1, HCV CO-infected individuals was associated with geographic areas and transmission routes.HCV subtypes had no direct correlation with HIV infection disease progression. |
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