| 曹敬荣,魏星,闫中强,沈定霞,罗燕萍.多重耐药鲍曼不动杆菌的分子流行特征分析[J].Chinese journal of Epidemiology,2009,30(8):832-835 |
| 多重耐药鲍曼不动杆菌的分子流行特征分析 |
| Study on the molecular characteristics of multidrug-resistant Acinetobacter baumannii |
| Received:January 21, 2009 |
| DOI： |
| KeyWord: 鲍曼不动杆菌 多重耐药 分子流行病学 |
| English Key Word: Acinetobacter baumannii Multidrug-resistant Epidemiology,molecular |
| FundProject:军队"十一五"课题(06MA294) |
| Author Name | Affiliation | E-mail | | CAO Jing-rong | Department of Microbiology, PLA General Hospital, Beijig 100853, China | | | WEI Xing | Department of Microbiology, PLA General Hospital, Beijig 100853, China | | | YAN Zhong-qiang | Department of Microbiology, PLA General Hospital, Beijig 100853, China | | | SHEN Ding-xia | Department of Microbiology, PLA General Hospital, Beijig 100853, China | shendx301@yahoo.com | | LUO Yan-ping | Department of Microbiology, PLA General Hospital, Beijig 100853, China | |
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| Abstract: |
| 目的 研究不同医院分离的鲍曼不动杆菌产碳青霉烯酶的基因型和分子流行特征.方法 采用琼脂稀释法检测64株多重耐药鲍曼不动杆菌对抗菌药物的最小抑菌浓度(MIC);PCR检测碳青霉烯酶基因、整合酶基因及per基因,选取不同的耐药克隆菌株进行序列测定;脉冲场凝胶电泳(PFGE)分析菌株同源关系,确定菌株的分子流行特征.结果 50株菌(78.1%)携带blaOXA-23-like基因,经测序分析确定为OXA-23;1株菌检测出bla,OXA-58-like基因;57株菌(89.1%)携带I类整合子;25株菌(39.1%)检测出blaPER-1基因.PFGE图谱显示64株菌分为A、B、C、D、E等13个基因型,三家医院分别以A型、B型和U型为主要流行株.结论 三家医院均发现多耐药鲍曼不动杆菌的播散流行,不同医院间和同一医院不同科室间存在同型别流行;三家医院多耐药鲍曼不动杆菌产生的碳青霉烯酶常携带OXA-23型酶,同时检测出blaOXA-58、I类整合子和blaPER基因. |
| English Abstract: |
| Objecfive To investigate antibiotic resistance,carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.Methotis The minimum inhibitory concentrations (MIC) were examined by ager dilution method.Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced.PCR was used to detect per gene,Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis(PFGE).Results Among the 64 MDRA strains,78.1%(50)strains possessed blaOXA-23 gene,89.1%(57) carried Class 1 integrase gene,39.1% (25) with blaPER-1 gene,and 1 strain with blaOXA-58-likegene.PFGE showed that 13(A,B,C,D,E genotype) different clones were identified in these strains.A,B,and U clones were the predominant clones in three hospitals,respectitively.Conclusion OutbreaEs of muitidrug-rcsistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme.OXA-58 type of carbapenemase and per-1 in Aba were also isolated. |
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