狂犬病毒中和抗体检测快速荧光灶抑制试验的建立

于鹏程; 吕新军; 申辛欣; 曹蕾; 郑新雄; 单虎; 唐青

Abstract

于鹏程,吕新军,申辛欣,曹蕾,郑新雄,单虎,唐青.狂犬病毒中和抗体检测快速荧光灶抑制试验的建立[J].Chinese journal of Epidemiology,2010,31(4):438-441

狂犬病毒中和抗体检测快速荧光灶抑制试验的建立

The establishment of a rapid fluorescent focus inhibition test for testing rabies virus neutralizing antibody

Received:September 28, 2009

DOI：10.3760/cma.j.issn.0254-6450.2010.04.018

KeyWord: 狂犬病毒|中和抗体|荧光灶抑制试验|滴度

English Key Word: Rabies virus|Neutralizing antibody|Fluorescent inhibition test|Titer

FundProject:公益性行业(农业)科研专项基金(200803014);
国家自然科学基金(306300492);
日本国家传染病研究(NIID)基金;
艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZXl0004-002)

Author Name	Affiliation	E-mail
Yu Pengcheng	Animal Science and Technology College, Qingdao Agriculture University, Qingdao 266109, China Institute for Viral Disease Control and Prevention of Chinese Center for Disease Control and Prevention
Lv Xinjun	Institute for Viral Disease Control and Prevention of Chinese Center for Disease Control and Prevention
Shen Xinxin	Institute for Viral Disease Control and Prevention of Chinese Center for Disease Control and Prevention
Cao Dong	Institute for Viral Disease Control and Prevention of Chinese Center for Disease Control and Prevention
Zheng Xinxiong	Wuhan Institute of Biologic Products
Shan Hu	Animal Science and Technology College, Qingdao Agriculture University, Qingdao 266109, China
Tang Qing	Institute for Viral Disease Control and Prevention of Chinese Center for Disease Control and Prevention	qtang04@sina.com

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Abstract:

目的建立检测狂犬病毒中和抗体的快速荧光灶抑制试验(RFFIT), 检测狂犬病毒中和抗体滴度。方法以狂犬病标准毒株CVS-11为标准攻击毒, 制备三代病毒, 建立CVS-11病毒库; 以国际标准抗狂犬病毒血清为对照血清, 参考法国巴斯德研究所的RFFIT操作标准, 建立适应于中国狂犬病毒中和抗体检测的RFFIT方法, 并验证该方法的特异性、稳定性和重复性。结果成功建立了检测狂犬病毒中和抗体的RFFIT试验, 验证该试验方法的特异性为100%; 重复性试验表明, 在本实验室和不同实验室的检测结果P值均>0.5, 具有很好的重复性。结论RFFIT方法的建立进一步完善了中国狂犬病的实验室检测技术, 也将提升中国狂犬病的整体监测能力。

English Abstract:

Objective To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody. CVS-11 was used as the standard challenge virus, and three generations prepared for the establishment of the virus library. Methods International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated. Results we established the RFFIT which showed both good specificity (100%) and reproducibility(P>0.5). Conclusion The establishment of RFFIT test perfected the rabies laboratory techniques and would enhanc the overall ability in detecting rabies in China.

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