对氧磷酶和谷胱甘肽S转移酶基因多态性与农药暴露花卉种植者的健康关系研究

何建辉; 刘苹; 漆骏; 杨红英

Abstract

何建辉,刘苹,漆骏,杨红英.对氧磷酶和谷胱甘肽S转移酶基因多态性与农药暴露花卉种植者的健康关系研究[J].Chinese journal of Epidemiology,2010,31(7):759-762

对氧磷酶和谷胱甘肽S转移酶基因多态性与农药暴露花卉种植者的健康关系研究

Relationship between paraoxonase/glutathione S-transferase gene polymorphism and health conditions of floriculture farmers

Received:December 29, 2009

DOI：

KeyWord: 健康效应花卉种植者基因多态性对氧磷酶谷胱甘肽S转移酶

English Key Word: Health effects Floriculturists Gene polymorphism Paraoxonase Glutathione S-ransferase

FundProject:云南省科学技术基金(2005GH20)

Author Name	Affiliation	E-mail
HE Jian-hui	Department of Environmental and Occupational Health, School of Public Health, Kunming Medical College, Kunming 650031, China
LIU Ping	Department of Environmental and Occupational Health, School of Public Health, Kunming Medical College, Kunming 650031, China	liupingkm@gmail.com
QI Jun	Yunnan Provincial Center for Disease Control and Prevention
YANG Hong-ying	The Second Affiliated Hospital of Kunming Medical College

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Abstract:

目的探讨花卉种植者(花农)对氧磷酶及谷胱甘肽S转移酶基因多态性的分布情况,并分析基因多态性与健康的关系.方法选择云南省某村136名从事花卉种植的花农作为暴露组(男性74名,女性62名);41名不使用农药的同村居民作为对照组(男性16名,女性25名);进行问卷调查和体格检查,并采集血液样本,分析血常规、肝功能、激素水平、免疫球蛋白(IgM、IgG、IgA)和全血乙酰胆碱酯酶活性.采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)检测PON1-107、PON1 192、GST M1、T1基因型.结果 (1)对照组球蛋白高于暴露组(β=-0.16,t=-2.30,P=0.02),而白蛋白与球蛋白的比(白球比)低于暴露组(β=0.14,t=2.09,P=0.04);(2)PON1 192位点分析显示,QQ基因型个体症状积分高于RR基因型个体(t=-2.78,P=0.006);(3)GSTT1-的个体分析显示,球蛋白高于GSTT1+的个体(β=-0.20,t=-3.01,P=0.00),IgG高于GST T1+的个体(β=-0.15,t=-2.15,P=0.03),而白球比低于GST T1+的个体(β=0.17,t=2.54,P=0.01);(4)农药暴露者心电图异常情况增加(β=1.147,P=0.042).结论 PON1 192和GST T1位点与农药暴露后的花农健康状况相关.

English Abstract:

Objective In this study we conducted a cross-sectional study and reported on the distribution of two common genetic polymorphisms of the PON1 gene and two common genetic polymorphisms of the GST gene as well as the association between those polymorphisms and other predictors in a population of floriculture workers from Kunming city.Methods 136 pesticide-exposed farmers were recruited. PON1 and GST T1, M1 genotype were determined by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results (1)Compared with exposure group, the GLB in control group was higher(β=-0.16,t=-2.30, P=0.02), but the A/G in control group was lower (β=0.14,t=2.09,P=0.04); (2) Compared with the persons who carried QQ genotype, the accumulative symptom scores were lower in the persons who carried RR genotype(t=-2.78, P=0.006); (3) Compared with GST T1 carriers, non-carriers' GLB (β=-0.20, t=-3.01, P=0.00)and IgG(β=-0.20,t=-3.01, P=0.00)were higher, but the A/G was lower; (4) The abnormities of cardiograms among people who had been exposed to pesticides were higher compared to people who did not expose to any pesticides (β=1.147, P=0.042). Conclusion PON1-192 and GST T1 gene were associated with the farmers health condition after pesticides exposure.

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