北京地区流行的HIV-1 CRF01_AE株基因特征研究

叶景荣; 卢红艳; 白立石; 辛若雷; 曾毅

Abstract

叶景荣,卢红艳,白立石,辛若雷,曾毅.北京地区流行的HIV-1 CRF01_AE株基因特征研究[J].Chinese journal of Epidemiology,2010,31(11):1231-1234

北京地区流行的HIV-1 CRF01_AE株基因特征研究

Genetic characteristics of HIV-1 CRF01—AE strains circulating in Beijing

Received:September 09, 2010

DOI：

KeyWord: 人类免疫缺陷病毒亚型巢式聚合酶链式反应

English Key Word: Human immunodeficiency virus Subtype Nested-PCR

FundProject:国家科技蓽大专项(2008ZX10001-009)

Author Name	Affiliation	E-mail
YE Jing-rong	National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China Beijing Center for Disease Control and Prevention	basketball197602@hotmail.com
LU Hong-yan	Beijing Center for Disease Control and Prevention
BAI Li-shi	Beijing Center for Disease Control and Prevention
XIN Ruo-lei	Beijing Center for Disease Control and Prevention
ZENG Yi	National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China

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Abstract:

目的分析北京地区HIV-lCRF01一AE株在的棊因特征。方法采集北京地区2006—2008年新确证HIV感染者的抗凝全血标本, 分离血浆和提取病毐RNA, 用反转录/巢式聚合酶链式反应扩增病毐gag基因, 对获得的105条CRF01_AE株序列进行系统进化分析, 并通过计算组间距离和Entropy核苷酸多态性差异方法分析病毒洙的遗传特征。结果在CRF01一AE株序列系统进化树中存在4个主要的亚簇, Homo-Max亚簇包含67例样本, 同参考株CM240组间基因离散率为5.4%, 组内离散韦为2.8%;Hetero亚簇包含6例样本, 组间基因离散率为4.8%, 组内离散率为4.2%。Mix亚簇包含8例样本, 组间基因离散率为4.2%, 组内离散率为5.2%。Homo-Min亚簇包含18例样本, 组间基因离散韦为4.4%, 组内离散率为2.8%。Homo-Max、Hetero和Homo-Min亚簇尤其他M家的序列, Mix亚簇与越南和泰HCRF01—AE株关系密切;核伴酸多态性分析结果S示, 以Mix亚簇为参照, Homo-Max、Homo-Min和Hetero亚族分别有37、29和11个位点的核苷酸组成, 差异有统计学意义。结论北京地区流行的CRH)1_AE病毒株中昏次观察到4个独立的亚簇；Homo-Max亚簇株为最主要的CRF01_AE株；Mix亚簇株与来自于泰M和越南的CRF01_AE株有较高同源性。

English Abstract:

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in Beijing. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals being reported during 2006 to 2008 in Beijing. Gag gene fragments were amplified from RNA template which were extracted from plasma by RT and nested PCR methods. 105 CRF01AE sequences were analyzed by phylogcnctic methods and characterized through calculating the genetic distance and Entropy analysis. Results There were four main sub-clusters in the phylogenetic tree.We named them as sub-clusters Homo-Max (67 sequences) t Hetero (6 sequences), Mix (8 sequences) and Homo-Min (18 sequences)respectively, based on the mode of transmission. It was found that no international reference strain was closely related to the sub-cluster Homo-Max, Hetero or Homo-Min, including 91 samples. The strains in sub-cluster Mix consisting 8 cases that were closely related to the strains identified in Thailand and Vietnam. Genetic distance analysis on gag genes showed that the diversity of sub-clusters Horno-Max and Homo-Min was obviously less than that of the sub-cluster Hetero or Mix. When compared with sub-cluster Mix, there were 37, 29 and 11 significantly different nucleotides polymorphism compositions sites in sub-clusers Homo-Max Homo-Min and Hetero.Conclusion This was the first report describing that four main epidemic sub-clusters were existed in CRF01_AE strains prevailing in Beijing. The virus with sub-cluster Homo-Max was the dominant strain in this region with shorter period of circulation and higher proportion seen in the HIV-infected persons. The virus in sub-cluster Mix was highly homologic with the CRF01AE strains from Thailand and Vietnam.

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