| 张慧娟,魏建春,张恩民,申小娜,梁莹,朱晓宇,徐冬蕾,孙丽娜,张建华,俞东征,海荣.鼠疫耶尔森菌rRNA基因识别特征研究[J].Chinese journal of Epidemiology,2011,32(4):366-369 |
| 鼠疫耶尔森菌rRNA基因识别特征研究 |
| Study on the identiffcation characteristies of dRNA genes On Yersinia pestis |
| Received:December 24, 2010 |
| DOI： |
| KeyWord: 鼠疫耶尔森菌 rRNA基因 识别特征 |
| English Key Word: rsiniapestis rRNAgenes Identification characteristics |
| FundProject:国家“十五”科技攻关项目(2004BA718807)；国家科技部重大专项(2008ZXl0004-008)；卫生部专项经费资助项目(200802016) |
| Author Name | Affiliation | E-mail | | Zhang Huijuan | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Wei Jianchun | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Zhang Enmin | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Shen Xiaona | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Liang Ying | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Zhu Xiaoyu | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Xu Donglei | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Sun Lina | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Zhang Jianhua | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Yu Dongzheng | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | yudongzheng@icdc．Cn | | Hai Rong | State Key Laboratoryfor Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | haimng@icdc.cn |
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| Abstract: |
| 目的分析鼠疫耶尔森菌(鼠疫菌)的rRNA基因识别特征。方法通过比较基因组学方法，利用Clustal W软件，对目前已完成全基因组测序的9株鼠疫菌的rRNA基因序列进行分析，分别比对rRNA基因簇两侧的2000bp序列、基因簇内16S、23S、5S rRNA及16S～23S基因间隔区序列，以确定鼠疫菌rRNA基凶的识别特征。结果菌株D182038、D106004、Z176003和C092分别有6个rRNA基因簇拷贝(6拷贝菌株)，菌株91001、KIM、Nepal516、Antiqua和PestoidesF分别有7个rRNA基因簇拷贝(7拷贝菌株)。按照两侧2000bp序列不同，可将鼠疫菌rRNA基因簇分成13种类型，并可将6拷贝与7拷贝菌株进行区分；16S一23S rRNA基因间隔区含有4种不同种类和数量的tRNA基因，可将6拷贝菌株和7拷贝菌株分别进行区分；23S rRNA基因在不同菌株间及不同rRNA拷贝间的碱基突变数方差分析显示，各菌株间F=0．548，P=0．815>0．05；各拷贝间F=5．228，P<0．01。结论鼠疫菌rRNA基因簇两侧序列、间隔区tRNA基因和23S rRNA基因可作为识别不同菌株和不同rRNA基因簇拷贝的指标，将鼠疫菌不同菌株进行分类。 |
| English Abstract: |
| Objective To study the identification characteristics of rRNA genes on Yeninia (K)pestis．Methods By means of comparative genomics，we compared the rRNA genome sequences of nine completely sequenced strains of M pestis isolated from China and other countries by Clustal W software．We alSO compared the 2000 bp sequence adjacent to the rRNA genes．rRNA genes and 16S一23S rRNA spacer region respectively to determine the identification features of rRNA genes for y．pestis．Results There were 6 rRNA gene clusters in the strains of D182038，D106004， Z176003 and C092 respectively(6 copies strain)．There were 7 rRNA gene clusters in the strains of 91001，ⅪM，Nepal516，Antiqua and Pestoides F(7 copies strain)．According to the 2000 bp sequence，13 types ofrRNA gene clusters could classify the strains between the 6 copies and 7 copies．There were 4 types of tRNA gene among the 16S-23S rRNA spacer region that could classify the trains among the 6 copies and 7 copies strains respectively．ne number of point mutation among the 23S rRNA gene was statistically different in some copies under ANOVA analysis(F=O．548，P=0．815>0．05 among the strains and F=5．228．PConclusion The 2000 bpsequence甜jacent to the rRNA genes，tRNA gene and 23S rRNA gene sequence could serve as the identification sign of rRNA genes for classifing the strains ofY pestis． |
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