| 马绍辉,施海晶,何春艳,陈俊英,杨卉娟,孙强明.云南省2007--2009年流行性腮腺炎病毒SH和HN基因的遗传特征分析[J].Chinese journal of Epidemiology,2011,32(4):370-375 |
| 云南省2007--2009年流行性腮腺炎病毒SH和HN基因的遗传特征分析 |
| Genetic characteristics on the small hydrophobic protein and hemagglutinin—neuraminidase genes of mumps virus in Yunnan province，China from 2∞7 to 2009 |
| Received:October 11, 2010 |
| DOI： |
| KeyWord: 腮腺炎病毒 F基因型 SH基因 HN基因 |
| English Key Word: Mumps virus F genotype SH gene HN gene |
| FundProject:云南省基础研究面上项日(2008CDl53) |
| Author Name | Affiliation | E-mail | | Ma Shaohui | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | shaohuima70@hotmail．com | | Shi Haijing | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | | | He Chunyan | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | | | Chen Junying | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | | | Yang Huijuan | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | | | Sun Qianming | Institute of Medical Biology. Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | |
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| Abstract: |
| 目的分析2007—2009年云南省腮腺炎病毒(MuV)分离株的SH和HN遗传特征。方法采用反转录一聚合酶链反应从Vero细胞分离的病毒株基因组中扩增出SH基因和HN基因，测序后用Mega 4．1软件分析其遗传特征。结果云南省分离14株MuV的sH基因其核苷酸和氨基酸同源性为98．3％一100．O％和96．5％～100．0％，与其他省份比较其同源性为92．6％一99．4％和87．7％一100．O％，其中Wshl和Wsh2与其他F基因型差异较大；与疫苗株同源性为84．5％一85．1％和77．2％；与其他基因型同源性为83．4％～90．9％和70．1％～86．0％。6株MuV分离株的l-IN基因与核苷酸和氨基酸同源性分别为99．3％～99．5％和99．1％一99．7％；与中国分离株sP株的核苷酸和氨基酸同源性均为99．8％；与其他基因型同源性为94．7％～96．8％和95．5％一99．1％；与疫苗株的同源性为92．4％～93．2％和95．5％一96．4％。结论2007--2009年云南省流行的MuV均为F基因型；其HN基因比SH基因保守。 |
| English Abstract: |
| Objective To analyze genetic characterization of the small hydrophobic and hemagglutinin．neuraminidase genes of mumps virus(MuV)isolatcd in Yunnan province。China from 2007 to 2009．Methods Fourteen MuV strains were isolated in Yunnan．C：hina from 2007 to 2009．Using RT-PCR．the SH gene fragments contained 3 l 6 nucleotides in all strains and HN gene of six strains were sequenced．The sequences were aligned with other mumps virus sequences downloaded from GenBank using Mega 4．1 software．Results Fourteen isolated strains were closely related to other reference strains of F genotypes．In SH gene。the homology of nucleotide and amino acid among the fourteen isolated strains were 98．3％一100．0％and 96．5％一100．0％．respectively。and 92．6％一99．4％and 87．7％一100．0％of homology when compared with that of strains isolated from other provinces in China，respectively．Wshl and Wsh2 strains had less homology when compared to other strains of F genotypcs．The fourteen strains had homology of 84．5％一85．1％and 77．2％compared to vaccine strains on nucleotide and amino acid.respectively．and had homology of 83．4％-90．9％and 70．1％-86．0％compared to that of other genotypes．In HN gene．the homology of nueleotide and amino acid among the six isolated strains were 99．3％-99．5％and 99．1％-99．7％，respectively，and also 99．8％and 99．8％of homology respectively when compared to the SP strain in China．All the six strains had homology of 92．4％-93．2％and 95．5％-96．4％when compared to the vaecine strains on nucleotide and amino acid．respectively。and had homology of 94．7％一96．8％and 95．5％一99．1％ compared to other genotypes．Conclusion Fourteen strains isolated in Yunnan from 2007 to 2009
belonged to Fgenotype of MuV while the HN gene seemed more conservative than SH gene． |
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