| 梁冰玉,庄道民,蒋俊俊,刘思扬,苏齐鉴,李敬云,梁浩.吗啡对拉米夫定抗HIV-1药效影响的体外MT2细胞实验研究[J].Chinese journal of Epidemiology,2011,32(7):705-708 |
| 吗啡对拉米夫定抗HIV-1药效影响的体外MT2细胞实验研究 |
| Antiviral effect of lamivudine on HIV—1 targeting MT2 cells influenced by morphine |
| Received:December 27, 2010 |
| DOI： |
| KeyWord: 艾滋病1型病毒 吗啡 拉米夫定 抑制率 |
| English Key Word: HlV—l Morphine Lamivudine Inhibition |
| FundProject:国家自然科学基金(30760218)；美国国立卫生研究院资助课题(I-R21一DA025477—01) |
| Author Name | Affiliation | E-mail | | LIANG Bing-yu | Guangxi Key Laboratory Cultivation Base of AlDs Prevention and Treatment＆Department of Epidemiology of School of Public Heahh, Guangxi Medical University, Nanning 530021, China | | | ZHUANG Dao-min | Institute ofMicrobiology and Epidemiology, Academy of Military Medical Sciences | | | JIANG Jun-jun | Guangxi Key Laboratory Cultivation Base of AlDs Prevention and Treatment＆Department of Epidemiology of School of Public Heahh, Guangxi Medical University, Nanning 530021, China | | | LIU Si-yang | Institute ofMicrobiology and Epidemiology, Academy of Military Medical Sciences | | | SU Qi-jian | Guangxi Key Laboratory Cultivation Base of AlDs Prevention and Treatment＆Department of Epidemiology of School of Public Heahh, Guangxi Medical University, Nanning 530021, China | | | LI Jing-yun | Institute ofMicrobiology and Epidemiology, Academy of Military Medical Sciences | | | LIANG Hao | Guangxi Key Laboratory Cultivation Base of AlDs Prevention and Treatment＆Department of Epidemiology of School of Public Heahh, Guangxi Medical University, Nanning 530021, China | haolphd@163．com |
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| Abstract: |
| 目的探讨吗啡是否影响拉米夫定(3Tc)抗HIV-1的抗病毒效果。方法MT2细胞随机分为吗啡+3TC、吗啡+纳洛酮+3TC、纳洛酮+3TC处理组及3TC对照组和病毒对照组；用纳洛酮处理相应组的MT2细胞0．5 h后，加入吗啡处理细胞24 h，然后每组细胞加入等量的HIV-ImB病毒和3TC溶液；病毒感染细胞第3、4、5和6天，取培养上清，用ELISA法检测培养上清的HIV-I p24抗原，根据p24抗原表达量，计算各个处理组的3TC抗HIV-l p24抗原抑制率。结果HIV—l感染细胞第3和第4天，吗啡+3TC处理组的3TC抗HIV-I p24抗原抑制率最低，与3TC对照组相比差异有统计学意义(P0．05)，这两组分别与3TC对照组相比，差异无统计学意义(P>O．05)；病毒感染细胞第5和第6天，各处理组的3TC抗HIV-1 p24抗原抑制率与3TC对照组相比，差异无统计学意义(P>O．05)；各个处理组的3TC抗HIV-1 p24抗原抑制率随感染时间延长而降低，呈现时间一效应关系。结论吗啡在病毒感染初期能降低3TC的抗HIV-I药效；纳洛酮能够阻滞吗啡降低3TC的抗HIV-1药效作用。 |
| English Abstract: |
| Objective To determine whether morphine having the ability to influence the antiviral efrect of lamivudine(3TC)in vitro study．Methods MT2 cells were randomly assigned into morphine+3TC treatment group，morphine+naloxone+3TC treatment group．naloxone+3TC treatment group．Both 3TC and vires control groups were set uD．The corresponding MT2 cells weretreated with opiates antagonist(naloxone)for 0．5 hours before the 24一hours morphine treatment program was implemented while all of the groups were then infected with equal amounts of cell．free HIV-1 ⅢB strain and 3TC．HIV=l p24 antigen in culture supernatants collected at days 3．4.5 and 6 after infection status was tested and the inhibition of 3TC anti．HIV-l p24 antigen of various treatment groups calculated．Results Inhibition of 3TC anti．HIV-l p24 antigen of Morphine+3TC treatment group was the lowest when HIV|-l infected cells at 3thand 4th day and showed significant difference (P<0．05)when compared to the 3TC contr01．HoweveT，there was no statistically significant difference among them(P>0．05)．when vires was infected the cells at 5thand 6thday．The difference of 3TC anti—HIV-l p24 antigen inhibition between the morphine+naloxone+3TC treatment group and the naloxone+3TC treatment group was not significant(P>0．05)．Similar results were obtained when these two groups wgre compared to the 3TC controI group(P>0．05)。respectively．The 3TCanti—HIV-l p24 antigen inhibition ofeach treatment group reduced as the time ofinfection prolonged． showing a significant and time—course effbct．Conclusion The 3TC antiviral effect was reduced by morphine in the early stage of infection．and could be blocked by naloxone． |
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