| 诸葛小玲,崔大伟,吴英萍,郑书发,靳淼,段招军,余斐,许世佳,陈凌晓,陈瑜.杭州地区2009-2010年急性腹泻患者中杯状病毒的检测和分型[J].Chinese journal of Epidemiology,2011,32(10):1022-1025 |
| 杭州地区2009-2010年急性腹泻患者中杯状病毒的检测和分型 |
| Detection and typing of caliciviruses from patients with acute diarrhea in Hangzhou area, 2009-2010 |
| Received:March 31, 2011 |
| DOI：10.3760/cma.j.issn.0254-6450.2011.10.016 |
| KeyWord: 杯状病毒 诺如病毒 札如病毒 基因型别 |
| English Key Word: Human caliciviruse Norovirus Sapovirus Genotype |
| FundProject:国家“十一五”科技重大专项( 2009ZX10004-210) |
| Author Name | Affiliation | E-mail | | ZHUGE Xiao-ling | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | | | CUI Da-wei | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | | | WU Ying-ping | Department of Clinical Laboratory, Yiwu Hospital, College of Medicine, Zhejiang University | | | ZHENG Shu-fa | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | | | JIN Miao | National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention | | | DUAN Zhao-jun | Department of Clinical Laboratory, Yiwu Hospital, College of Medicine, Zhejiang University | | | YU Fei | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | | | XU Shi-jia | Department of Clinical Laboratory,Pinghu First People's Hosptial | | | CHEN Ling-xiao | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | | | CHEN Yu | Department of Clinical Laboratory, the First Affiliated Hospital,College of Medicine, Zhejiang University, Hangzhou 310003, China | chenyu_zy@163.com |
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| Abstract: |
| 目的 了解2009-2010年杭州地区急性腹泻患者中杯状病毒基因型别和分子流行病学特征。方法 收集2009-2010年杭州地区920例急性腹泻患者的粪便标本和流行病学资料，用多重PCR方法进行杯状病毒检测，测定部分标本的阳性扩增片段基因序列，并对序列进行系统进化分析。结果 急性腹泻患者杯状病毒检出率为21.8%(201/920)，其中诺如病毒(NV)G Ⅰ型 25例、GⅡ型170例、札如病毒(SAV) 11例，4例为NVⅠ型和Ⅱ型混合感染、1例为NVⅡ型和SAV 混合感染。NV基因型别包括：GⅠ-1（3株）、G Ⅰ-2（1株）、GⅡ-4/2006b变异株（7株）、GⅡ-2(1 株)、GⅡ-7（1株）和GⅡ-4/2008变异株（2株）；SAV基因型别包括：G Ⅰ -2（5株）、GⅠ-1(4株)和 GⅡ-1（1株）。杯状病毒的流行在不同季节、年龄组人群均有发病。结论 杯状病毒是2009- 2010年引起杭州地区急性腹泻的主要病原之一，其病原具有病毒多样性和基因型别多样性，NVGⅡ-4/2006b变异株或类似株可能是2009-2010年杭州地区流行的优势株。 |
| English Abstract: |
| To investigate the molecular-epidemiologic characteristics and genotypes of human calicivirus (HuCVs) in acute diarrhea patients in Hangzhou from 2009 to 2010. Methods Epidemiologic data and fecal specimens were collected from patients with acute diarrhea. HuCVs of 920 specimens were detected by PCR. PCR products of several positive samples were randomly selected and sequenced. All the sequences were analyzed, phylogenetically. Results 201 HuCVs positive cases were identified from 920 facal specimens (21.8%). 25 isolates would include norovims G Ⅰ -type, G Ⅱ -type for 170 strains and sapovirus for 1 1 strains. Norovirus G Ⅰ -type and G Ⅱ -type were detected in four specimens at the same time. Other specimens were mixed infection with norovirus G Ⅱ -type and sapovirus. Genotypes of HuCVs showed that norovirus G Ⅰ subtypes were G Ⅰ -1 (3 strains) and G Ⅰ -2 (1 strain). Norovirus G Ⅱ subtypes were G Ⅱ -4/2006b variant strains (7 strains), GⅡ-2 (1 strain), G Ⅱ -7 (1 strain) and G Ⅱ -4/2008 variant strains (2 strains) ; Sapovirus subtypes were G Ⅰ -2 (5 strains) ,G Ⅰ -1 (4 strains) and G Ⅱ-1 ( 1 strain). The prevalence rates of HuCVs were different in seasons and age groups. Conclusion HuCVs were one of the major pathogens causing acute diarrhea. Both multiple viruses and genotypes of HuCVs were found in the specimens. G Ⅱ-4/2006b variant and similar strains were identified, probably as the prevalent strains from 2009 to 2010 in Hangzhou, Zhejiang province. |
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