江苏省260株结核分枝杆菌Spoligotyping基因分型研究

刘巧; 许卫国; 万康林; 王建明; 杨丹丹; 吕冰; 邵燕; 汪华

Abstract

刘巧,许卫国,万康林,王建明,杨丹丹,吕冰,邵燕,汪华.江苏省260株结核分枝杆菌Spoligotyping基因分型研究[J].Chinese journal of Epidemiology,2011,32(12):1250-1254

江苏省260株结核分枝杆菌Spoligotyping基因分型研究

Study on the genotypes of 260 Mycobacterium tuberculosis isolates by Spoligotyping method in Jiangsu province, China

Received:June 28, 2011

DOI：

KeyWord: 结核分枝杆菌|基因分型|北京家族

English Key Word: Mycobacterium tuberculosis| Genotyping| Beijing family

FundProject:国家科技重大传染病专项(2008ZX 10003-010);国家科技重大专项(2009ZX10004-904)

Author Name	Affiliation	E-mail
LIU Qiao	Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 210029, China	hua@scdc.cn
XU Wei-guo	江苏省疾病预防控制中心慢性非传染病防治所
WAN Kang-lin	State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control a
WANG Jian-ming	Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 210029, China
YANG Dan-dan	江苏省疾病预防控制中心慢性非传染病防治所
LV Bing	State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control a
SHAO Yan	江苏省疾病预防控制中心慢性非传染病防治所
WANG Hua	Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 210029, China

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Abstract:

目的研究江苏省结核分枝杆菌DNA指纹图谱的分布特征,分析北京家族株与结核分枝杆菌耐药的关联性.方法江苏省30个耐药监测点收集260株结核分枝杆菌分离株,应用比例法检测分离株对于一线抗结核药物(异烟肼、链霉素、利福平和乙胺丁醇)的耐药性,应用间隔区寡核苷酸分型(Spoligotyping)方法进行基因分型,使用BioNumerics5.0软件进行聚类分析,并与SpolDB4数据库比对.结果260株结核分枝杆菌分离株可分为34个基因型(27个独特基因型和7个共享基因型),菌株经聚类分析分为两个家族:北京家族(80.4％,209/260)和非北京家族(19.6％,51/260).logistic回归分析显示,北京家族株能增加结核分枝杆菌耐多药的发生风险(OR=11.07,95％CI:1.45～84.50).非北京家族包括T1、T2、H3、H4、CAS、LAM、U和MANU2,其中CAS、LAM和MANU2家族在中国比较罕见,在江苏省是首次报道.结论江苏省结核分枝杆菌流行株具有明显的基因多态性,其主要流行型为北京家族,且该家族可能与结核分枝杆菌耐多药之间存在关联.

English Abstract:

Objective To study the genotypes of Mycobacterium tuberculosis(M.tuberculosis)strains isolated from Jiangsu province and to explore the relationship between the 'Beijing family' and the drug resistance of M.tuberculosis.Methods Two hundred and sixty M.tuberculosis strains were isolated from 30 drug surveillance sites in Jiangsu province.Susceptibility of the isolates to the first-line antituberculosis drugs(isoniazid,streptomycin,rifampicin and ethambutol)was tested by using the proportion method.Molecular typing of M.tuberculosis strains was determined by Spoligotyping and analyzed with BioNumerics software.Results Based on Spoligotyping fingerprint,260 strains showed 34 different genotypes,including 27 exclusive genotypes and 7 shared genotypes.These strains could be clustered into two groups:the Beijing family(80.4％,209/260)and the Non-Beijing family(19.6％,51/260).Data from logistic regression analysis revealed that infection by the Beijing family was related to an increased risk of multi-drug resistant M.tuberculosis,with the OR(95％ CI)of 11.07(1.45-84.50).Non-Beijing families including T1,T2,H3,H4,CAS,LAM,U and MANU2 families were also found.Among them,the CAS,LAM and MANU2 families were first reported in Jiangsu province.Conclusion It was revealed that the marked gene polymorphisms did exist in M.tuberculosis strains.The Beijing family had been the predominant strain circulating in Jiangsu province,which might be related to multi-drug resistant M.tuberculosis strains.

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