非O157产志贺毒素大肠埃希菌分离株脉冲场凝胶电泳分析

金东; 赵爱兰; 白向宁; 孟琼; 于波; 袁雪娇; 熊衍文; 侯雪新; 李振军

Abstract

金东,赵爱兰,白向宁,孟琼,于波,袁雪娇,熊衍文,侯雪新,李振军.非O157产志贺毒素大肠埃希菌分离株脉冲场凝胶电泳分析[J].Chinese journal of Epidemiology,2013,34(5):503-506

非O157产志贺毒素大肠埃希菌分离株脉冲场凝胶电泳分析

Pulsed-field gel electrophoresis typing on non-0157 Shiga toxin-producing Escherichia coli isolates

Received:December 17, 2012

DOI：10.3760/cma.j.issn.0254-6450.2014.02.020

KeyWord: 非0157产志贺毒素大肠埃希菌脉冲场凝胶电泳分子分型

English Key Word: on-0157 Shiga toxin—producing Escherichia coli Pulsed—field gel electrophoresis Molecular typing

FundProject:家科技重大号项(2011ZXl0004—001，2012ZXl0004—501);卫生科研行业专项(201302006)

Author Name	Affiliation	E-mail
JlN Dong	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
ZHAo Ai lan	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
BAl Xiang ning	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
MENG Qiong	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
YU Bo	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
YUAN Xuejiao	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
XIONG Yanwen	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
HOU Xuexin	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China
LI Zhenjun.	State Key Laboratory for ln[ectious Disease Preyention and Control, National Institute for Communicable Disease Control and Prevention．Chinese Center for Disease Control and Prevention．Beiiing l02206．China	lizhenjun@icdc．Cn

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Abstract:

目的对国内不同宿主来源的非0157产志贺毒素大肠埃希菌(STEC)分离株进行脉冲场凝胶电泳(PFGE)分析，以初步建立中国非0157 STEC菌株的基础数据库，了解其分子流行病学特征。方法参照PulseNet非0157 STEC的PFGE实验方法对76株非0157 STEC分离株进行分析，并使用BioNumerics软件进行聚类。结果在PulseNet推荐的电泳参数和内切酶XbaI情况下，菌株酶切片段分布均匀，条带易于识别。76株非0157 STEC分离株产生62种PFGE带型，初步聚类为A～M 13个群，不同来源菌株的PFGE带型分布广泛，但均具有某些优势的PFGE聚类群。结论国内不同来源的非0157 STEC呈高度多态性，PulseNet推荐的PFGE电泳参数和内切酶XbaI适用于中国非0157 STEC菌株的分析。

English Abstract:

Objective To establish a database and to understand the molecular epidemiological features of non-Ol57 Shiga toxin．producing Escherichia coli(STEC)isolates from difrerent animal reservoirs and patients．Methods Pulsed—field gel electrophoresis(PFGE)was performed according to the PulseNet protocol with minor modifications．A dendrogram was constructed using the BioNumeriCS．Results Under the PulseNet protoc01．62 PFGE paRerns were obtained from 76 non．O 1 57 STEC isolates and then divided into A to M groups．Isolates from different sources were widely distributed in different groups．but were predominant seen in certain groups． Conclusion The non-O157 STEC isolates in China were highly polymorphic．PulseNet protocol seemed to be suitable for the typing of Chinese non—O157 STEC isolates．

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