贵州省-起人间布鲁氏菌病疫情的菌株鉴定及遗传特征分析

李世军; 王月; 王定明; 田克诚; 刘英; 马青; 刘昭宾; 龚晓俊; 唐光鹏; 陈贵春

Abstract

李世军,王月,王定明,田克诚,刘英,马青,刘昭宾,龚晓俊,唐光鹏,陈贵春.贵州省-起人间布鲁氏菌病疫情的菌株鉴定及遗传特征分析[J].Chinese journal of Epidemiology,2013,34(7):717-720

贵州省-起人间布鲁氏菌病疫情的菌株鉴定及遗传特征分析

Identification and genetic characteristics on the bacteria isolate from a case of human Brucellosis in Guizhou province

Received:January 28, 2013

DOI：10.3760/cma.j.issn.0254-6450.2013.07.013

KeyWord: 布鲁氏菌聚合酶链反应多位点可变数目串联重复序列

English Key Word: Brucellosis Polymerase chain reaction MLvA-16

FundProject:贵州省高层次人才科研条件特助经费项目(TZJ-2010-100)

Author Name	Affiliation	E-mail
LI Shi-jun	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China	zjumedjun@163.com
WANG Yue	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
WANG Ding-ming	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
TIAN Ke-cheng	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
LIU ying	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
MA Qing	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
LIU Zhao-bin	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
GONG Xiao-jun	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
TANG Guang-peng	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China
CHEN Gui-chun	Guizhou Provincial Center for Disease Control and Prevention.Guiyang 550004.China

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Abstract:

目的鉴定贵州省-起人间布鲁氏菌病(布病)疫情来源菌株(GZZA)并分析其遗传特征。方法应用传统方法和聚合酶链反应(PCR)鉴定菌株, 采用多位点可变数目串联重复序列(MLVA)-16分析其遗传特征。结果菌株(GZZA)采用传统方法和PCR鉴定为羊种生物3型布鲁氏菌, MLVA-16分析显示该菌株与布鲁氏菌各型代表菌株中的羊种生物3型布鲁氏菌聚类最近, 但在重复序列(VNTR)位点bruce42、bruce04、brace09和brucel6与羊种生物3型布鲁氏菌存在重复数目差异。结论该起疫情来源菌株(GZZA)鉴定为羊种生物3型布鲁氏菌, 其遗传特征与羊种生物3型菌株最接近, 但部分VNTR位点存在重复数目差异。

English Abstract:

ObjectiveA suspected Brucella(B.)strain(GzzA), isolated from a case of anti.Brucella antibody positive patient was identified and its'genetic characteristics was analyzed.to provide etiologic basis for the confirmation of patient in Guizhou province.Methods Conventional methods and polymerase chain reaction(PCR)were used to identify the bacteria strain.with genetic characteristics analyzed by MLVA-l6.ResultsThe bacteria strain was identified as B.melitensis biovar 3 under the conventional and PCR methods.Results from the MLVA-16 analysis indicated that the bacteria strain was closely clustered with B.melitensis biovar 3.and differences of repeated numbers at VNTR lOCi bruce42.bruce04.bruce09 and bruce 16 were also displayed.ConclusionBoth traditional and molecular methods to identify one bacteria strain isolated from the human patient as B.melitensis biovar 3 and the genetic characteristics of the strain was closely related to that of B.melitensis biovar 3.Difierences of repeated numbers at part of VNTR loci were also showed.The results of this study provided etiologic evidences for the confirmation of Brucella irifection of the patient.also providing scientific basis for the control and prevention of Brucellosis in Guizhou province．

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