扬州地区腹泻型肠易激综合征与HTR3A、HTR3E基因多态性关联研究

张瑜; 黄瑶; 卜平

Abstract

张瑜,黄瑶,卜平.扬州地区腹泻型肠易激综合征与HTR3A、HTR3E基因多态性关联研究[J].Chinese journal of Epidemiology,2013,34(7):721-724

扬州地区腹泻型肠易激综合征与HTR3A、HTR3E基因多态性关联研究

Association between diarrhea.predominant irritable bowel syndrome and HTR3A, HTR3E gene polymorphism in Yangzhou．Jiangsu province, China

Received:January 22, 2013

DOI：10.3760/cma.j.issn.0254-6450.2013.07.014

KeyWord: 腹泻型肠易激综合征基因多态性 HTR3A HTR3E

English Key Word: Diarrhea-predominant irritable bowel syndrome Gene Polymorphism HTR3A

FundProject:江苏省研究生创新工程项目(CXZZl2-0918);国家自然科学基金(8173392)

Author Name	Affiliation	E-mail
ZHANG YU	Medicaf College, Yangzhou University, Yangzhou 225009, China	yizhangyu@yzu.edu.cn
HUANG Yao	Yangzhou Center for Disease Control and Prevention
BO Ping	Medicaf College, Yangzhou University, Yangzhou 225009, China

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Abstract:

目的探讨扬州地区门诊腹泻型肠易激综合征(D.IBS)与5-羟色胺受体3的亚型HTR3A、HTR3E基因非翻译区多态性的关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR.RFLP)对450例D.IBS患者与300例健康对照者HTR3A基因5'端非翻译区C.-42C>T(rsl062613)和HTR3E基因3'非翻译区e.+76G>A(rs62625044)进行研究。结果男女性患者C.--42C>T多态性位点基因型分布较正常对照的差异有统计学意义(PA多态性位点G/A基因型和A等位基因的频率较正常对照均显著升高(P<0.05), 男性两组间的差异无统计学意义(P>0.05)；与HTR3A基因的rsl062613位点CC基因型及HTR3E基因的rs62625044位点GG基因型相比, TT基因型及GA基因型与D-IBS密切相关, 风险系数分别为0.29(95%CI：0.14～0.61)、0.62(95%CI：0.39。0.99), 差异均有统计学意义。结论T等位基因和GA基因型可能分别是D.IBS患者和D.IBS女性患者的易感因素之-。

English Abstract:

ObjectiveTo investigate the relationship between diarrhea-predominant irritable bowel syndrome(D-IBS)and HTR3A, HTR3E gene polymorphism in Yangzhou, Jiangsu province.MethodsPolymerase chain reaction(PCR)amplification and restriction fragment length pdymorphism(RFLP)technique were used to detect the 5'-UTR variant c.-42C>T of HTR3A and 3 7-UTR variant C.+76G>A of HTR3E on 300 healthy subjects and 450 patients with D-IBS.ResultsThere was significant difference noticed between the D-IBS patients and the controls in the gene type of C.-42C>T sites(P<0.05), while the frequency of T allele was significantly higher in both female and male patients than that in the controls(P<0.05).The frequencies of G/A gene type and A allele of C.+76G>A sites were significantly higher in the female-patient group than that in the controls(P<0.05), however, there was no significant difference between those male patients and controls(P>0.05).When comparing to the CC gene type of rsl062613 site and GG gene type of rs62625044 site.the TT and GA gene type were closely related to the D-IBS, with the risk coefficients as 0.29(95%CI:0.14-0.61)and 0.62(95%C/:0.39-0.99), with statistically significant differences.ConclusionT allele and GA gene type might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients．

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