| 胡守奎,郑田利,胡万富,王建军,李伟,崔志刚,周海健,逢波,阚飙.PulseNet China数据库在安徽省4起霍乱 疫情溯源中的应用[J].Chinese journal of Epidemiology,2013,34(10):1003-1005 |
| PulseNet China数据库在安徽省4起霍乱 疫情溯源中的应用 |
| Application of PlllseNet China Database in tracking and warning 4 cholerae outbreaks in Anhui province，in 2012 |
| Received:May 22, 2013 |
| DOI：10．3760/cma．j．issn．0254—6450．2013．10．014 |
| KeyWord: 中国细菌性传染病分子分型实验室监测网络|霍乱|预警 |
| English Key Word: PulseNetChina|Cholerae|urveillance |
| FundProject: |
| Author Name | Affiliation | E-mail | | HU Shou-kui | Anhui Center for Disease Control and Prevention. Hefei 230061, China
| | | ZHENG Wan-li | College of Public Health, Sichuan University
| | | HU Wan-fu | Anhui Center for Disease Control and Prevention. Hefei 230061, China
| | | WANG Jian-jun | Anhui Center for Disease Control and Prevention. Hefei 230061, China
| wjj@ahcdc.com | | LI Wei | State Key Laboratory br Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Centerfor Disease Control and Prevention | | | CUI Zhi-gang | State Key Laboratory br Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Centerfor Disease Control and Prevention | | | ZHOU Jian-hai | State Key Laboratory br Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Centerfor Disease Control and Prevention | | | FENG Bo | State Key Laboratory br Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Centerfor Disease Control and Prevention | | | KAN Biao | State Key Laboratory br Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Centerfor Disease Control and Prevention | |
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| Abstract: |
| 目的通过PulseNetChina数据库对安徽省2012年4起霍乱疫情进行溯源分析。 方法应用PCR扩增霍乱弧菌CT和TCP毒力基因，并利用脉冲场凝胶电泳(PFGE)对其进行分 型，使用BioNumericsV4．0软件对电泳结果进行聚类分析，并与PulseNet China数据库进行比对。 结果16株菌经PCR扩增，cT和TCP基因均出现目的条带。菌株经Not I和踬I酶切，PFGE聚 类分析显示，13株菌的同源性>98％。其中2株菌与PulseNetChina数据库中湖南、四川、浙江、上 海、湖北5省(市)同年霍乱疫情菌株同源性达100％。结论安徽省2012年4起霍乱疫情均源自 食用霍乱弧菌感染甲鱼。PulseNet China数据库能有效、快速地对霍乱疫情溯源和预警。 |
| English Abstract: |
| Objective To ffack the source of infection regarding 4 Cholerae outbreaks in Anhui province in 2012 through the applicaton of PulseNet China Database PNCD．Methads Cholerae virulence genes were amplified by PCR and typed by pulse field gel electrophoresis(PFGE)． Results from electrophoresis were cluster-analyzed by BioNumericsV4．0 software and compared wiⅡl PNCD．Results Virulence gene CT and TCP of the tested vibrio cholera showed both positive． Homology of the strains from four cholera outbreaks was more than 98％．based on the homologious and cluster analysis through enzyme digested PFGE electrophoresis．Those strains were highly homologous with the cholera epidemic strains identified in Hunan，Sichuan，Zhejiang，Shanghai and Hubei by PNCD．with the homology as 100％Conclusion Four chorela outbreaks in mnhui province．20 l 2 were highly correlated with the outbreaks occurring in Hunan and Sichuan during the same time period．indicating that PNCD could effectively and quickly tracking down the source of infection on the cholera outbreaks and providing early warning of the situation． |
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