乙醇代谢酶类基因多态性及环境暴露 在结直肠癌发生中交互作用的 单纯病例研究

赵浩; 李婷婷; 尹洁云; 秦芹; 师芸; 田丽红; 聂绍发; 汪鑫; 刘莉

Abstract

赵浩,李婷婷,尹洁云,秦芹,师芸,田丽红,聂绍发,汪鑫,刘莉.乙醇代谢酶类基因多态性及环境暴露在结直肠癌发生中交互作用的单纯病例研究[J].Chinese journal of Epidemiology,2013,34(10):1013-1017

乙醇代谢酶类基因多态性及环境暴露在结直肠癌发生中交互作用的单纯病例研究

Role of alcohol-metabolizing enzymes gene polymorphisms and environmental exposure on colorectal cancer：a case-only study

Received:May 10, 2013

DOI：10．3760/cma．j．issn．0254-6450．2013．10．017

KeyWord: 结直肠癌|基因一环境交互作用|基因一基因交互作用|单纯病例研究

English Key Word: Colorectal cancer|Oene．environment interaction|Gene-gene interaction|Gene-gene interactiononly study

FundProject:国家自然科学基金(30972534)

Author Name	Affiliation	E-mail
ZHAO Hao	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China School of Public Health, Nanchang Universit
LI Ting-ting	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
YIN Jie-yun	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
QIN Qin	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
SHI Yun	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
TIAN Li-hong	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
NIE Shao-fa	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
WANG Xin	Medical College of Jiujiang University	wangxin0072@126.com
LIU Li	Department of Epidemiology and Biostatistics, School of Publ Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China	gracefulliuly@163.com

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Abstract:

目的研究环境因素与乙醇脱氢酶lB(ADHlB)rsl229984、乙醛脱氢酶2(ALDH2) rs671、细胞色素P4502E1(CYP2E1)rsl329149交互及基因一基因之间交互在结直肠癌发生中的作用，分析该3个基因位点对结直肠癌发展的影响。方法采用单纯病例研究调查472例结直肠癌患者环境暴露情况和病理分期，用SequenomMassARRAY系统检测ADHlB(rsl229984)、ALDH2 (rs671)、CYP2E1(rsl329149)的基因型，应用非条件logistic回归分析ADHlB、ALDH2、CYP2E1基因多态性与环境因素及基因一基因之间的交互作用，采用?c2检验及非条件logistic回归评估 rsl229984、rs671、rsl329149与结直肠癌转移风险的关系。结果体重指数(BMI)与ADHlB rsl229984在结直肠癌发生中存在相乘交互作用(OR=1．720，95％C1：1．038。2．848，0‰=1．785， 95％CI：1．061～3．002)；曾经或现在吸烟与ADHlB(rSl229984)的基因多态性在结直肠癌发生中存在交互作用，经调整性别、年龄和饮酒后，该交互作用消失(OR=O．597，95％CI：0．387～0．921， OR．o=O．922，95％CI：0．509—1．669)；rsl229984、rs671、rsl329149与结直肠癌的转移风险无关。结论超重(BMI≥25 kg/m2)且携带ADHlB rsl229984 G易感等位基因可能增加结直肠癌发生的危险性，rsl229984、rs671、rsl329149与结直肠癌的转移无关。

English Abstract:

Objective This study was designed to explore the interactions of alcohol dehydrogenase 1B(ADHIB)rsl229984，aldehyde dehydrogenase 2(ALDH2)(rs671)and cytochrome P4502El(CYP2E1)rsl329149 with environmental factors and the interactions between genetic factors in the susceptibility of colorectal cancer(CRC)．Roles of genetic factors in the development of colorectai cancer were also studied．Methads With a case-only study design，472 colorectal cancer cases were enrolled between 2007 and 2009 in this study．Data on demographic characteristics．histories of environmental exposure and clinico-pathological parameters were obtained from all the participants through written questionnaires．Genotypes were determined by Sequenom MassARRAY system．Unconditional logistic regression analysis was employed to explore the gene．environment interactions and gene—gene interactions．)C2 test and unconditional logistic regression were used to evaluate the roles of polymorphisms on the risk of metastasis to CRC．Results Overweighted individuals that carrying at least one of the ADHlB rsl229984 G alleles presented significant increase on the risk to colorectal cancer(OR=1．720，95％CI：1．038-2．848 95％CI：1．061—3．002)．Modest interaction was seen between smoking and ADHlB(rsl229984)only before the adiustment of data，by sex，age and drinking status(OR=O．597，95％C/：0．387—0．921， O/L,=O．922．95％CI：O．509—1．669)．Correlations between polymor phisms andtheDukes stagewerenot found．Conclusion Overweight presented significant interaction with G allele ofADHlB rsl229984 in the susceptibility of CRC．None of the rsl229984，rs671 and rsl329149 exhibited significant influence on the development of CRC．

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