2011年河南省脑炎患者柯萨奇病毒B5型分离株基因组序列分析

马红霞; 潘静静; 康锴; 谢志强; 茹维萍; 陈豪敏; 黄学勇; 许汴利

Abstract

马红霞,潘静静,康锴,谢志强,茹维萍,陈豪敏,黄学勇,许汴利.2011年河南省脑炎患者柯萨奇病毒B5型分离株基因组序列分析[J].Chinese journal of Epidemiology,2013,34(12):1213-1215

2011年河南省脑炎患者柯萨奇病毒B5型分离株基因组序列分析

Genome sequences of coxsackievirus B5 isolated from viral encephalitis patients in Henanprovince,2011

Received:August 01, 2013

DOI：10.3760/cmaj.issn.0254-6450.2013.012.013

KeyWord: 柯萨奇病毒B5型脑炎系统进化分析

English Key Word: Coxsackievirus B5 Encephalitis Phylogenetic analysis

FundProject:河南省科技厅项目(122400450357);河南省医学科技攻关计划省部共建项目(20120100

Author Name	Affiliation	E-mail
Ma Hongxia	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Pan Jingjing	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Kang Kai	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Xie Zhiqiang	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Ru Weiping	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Chen Haomin	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Huang Xueyong	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China
Xu Bianli	Henan Provincial Center for Disease Control and Prevention Zhengzhon 450016, China	xubl@hncdc.com.cn

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Abstract:

目的分析河南省柯萨奇病毒B5(cvBs)分离株全基因序列,了解其遗传特性。方法采集2例脑炎患者临床标本,分离病毒,提取病毒阳性分离物RNA,进行RT-PCR扩增,产物直接测序。利用DNAStar 5.01和Mega 5.05软件进行全基因序列拼接及亲缘进化分析。结果获得2株CVB5分离株(03001N和17Y)全基因组序列,核苷酸长度分别为7408bP和7404 bP,两者核苷酸同源性为97%,同2010年河南CVB5分离株同源性最高,分别为98%和99%。5-UTR分别为747 bP和743 bP<；3-UTR区均为103 bP；病毒基因组编码区全长同为6558 bP,编码含2185个氨基酸残基的多聚蛋白,氨基酸同源性为99%。VPl区系统发生树分析显示,2株CVB5分离株进化关系较近,同近年来国内其他分离株成一簇,而2009年的河南省CVB5分离株同山东省CVB5分离株成一簇。结论河南省内存在不同CVB5株传播链的流行,此次分离株为近几年内的主要流行株。

English Abstract:

Objective To analyze the complete genome sequences of two coxsackievirus B5(CVB5)isolated in Henan province.201 1.Methods Specimens were collected from viralencephalitis patients and followed by vimi iso/at/on on them.RNA were extracted from positiveisolates and the amplified products were sequenced.The full.1ength genomes of them were acquiredby assembling the fragments.using DNAStar 5.01 software while phylogenetic analysis wereperformed with Mega 5.05 and other software.Results The genomes RNA of 0300 IN and l7Yshowed 7408 bp and 7404 bp long，and the 5 7-and 3’untranslated regions were 747 bp，743 bp and103 bp，103 bp，respectively.BLAST analysis of these two isolates，based on the complete genome，showed 97%identity，with bom of them having the highest similarity(98%，99%)to the CⅧ5strainisolated from Henan in 2010 rather than other CVB5 strains.Coding regions of both isolates were6558 bp，code for a polyprotein of2185 amino acids(aa)and both ofthem showed 99%amino acididentity.Phylogenetic tree m VPi region showed that the two isolates belonged to the same clade withother strains isolated from a11 over the country in the past years.except for some CVB5 strains isolatedfrom Henan and Shandong province in 2009 that formed the other cluster.Conclusion It seemed thatmore than one group of CVB5 were circulating in Henan province and these two isolates appeared themain idemic stra.ins circulating in the past years.

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