PTPRDrs2279776及其与HBV变异的交互作用对肝细胞癌风险的影响

邓阳; 张琪; 张玉伟; 韩雪; 曹广文

Abstract

邓阳,张琪,张玉伟,韩雪,曹广文.PTPRDrs2279776及其与HBV变异的交互作用对肝细胞癌风险的影响[J].Chinese journal of Epidemiology,2013,34(12):1228-1232

PTPRDrs2279776及其与HBV变异的交互作用对肝细胞癌风险的影响

Effect of PTPRD rs2279776 gene and interaction with hepatitis B virus mntations on the risk ofhepatoceHular carcinoma

Received:August 14, 2013

DOI：10.3760/cmaj.issn.0254-6450.2013.012.017

KeyWord: 肝细胞癌乙型肝炎病毒 PTPRD基因单核苷酸多态性变异交互作用

English Key Word: Hepatocellular carcinoma Hepatitis B virus PTPRD gene Single uucleotidepolymorphism Mutations Interaction

FundProject:国家自然科学基金(91129301);国家杰出青年基金(81025015);上海市自然科学基金(12ZRl453600)

Author Name	Affiliation	E-mail
Deng Yang	Department of Epidemiology, Second Military Medical University, Shanghai 200433,China
Zhang Qi	Department of Epidemiology, Second Military Medical University, Shanghai 200433,China
Zhang Yuwei	Department of Epidemiology, Second Military Medical University, Shanghai 200433,China
Han Xue	Yangpu District Center for Disease Control and Prevention, Shanghai
Cao Guangwen	Department of Epidemiology, Second Military Medical University, Shanghai 200433,China	gcao@smmu.edu.cn

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Abstract:

目的探讨PTPRD遗传多态性rs2279776及萁与HBV变异的交互作用和肝细胞癌(HCC)发生风险的关联性。方法采用实时定量PCR对1012例健康对照(对照组)、990例非肝细胞癌HBV感染者(非癌HBV感染组)及1021例乙型肝炎后肝细胞癌患者(HCC组)进行PTPRDrs2279776多态性检测,应用PCR测序法分别测定HBV核心启动子区及前s区变异。采用多因素logistic回归分析rs2279776、HBV变异及它们之间的交互作用和HCC发生风险的关联性。结果 rs2279776基因型和等位基因频率的分布在HCC组与对照组之间、HCC组与非癌HBV感染组之间以及HCC组与非癌组(非癌HBV感染组+对照组)之间的差异均无统计学意义。而rs2279776GC基因型与HBV变异T1753V和PreS缺失的交互作用显著增加女性HBV感染者患HCC的风险。rs2279776 GC基因型与HBV G1896A变异的交互作用可以降低HBV基因型B感染者患HCC的风险；CC基因型与HBVAl652G变异的交互作用可以显著降低基因型CHBV感染者息HCC的风险。结论 PTPRD rs2279776与HCC易感性无直接相关性,但可通过与HBV变异的交互作用对HCC发生风险产生影响。

English Abstract:

Objeetive To investigate the efrect of rs2279776 at the PTPRD and itsinteractions on hepatitis B virus(HBV)mutations as well as related risk on hepatocellular carcinoma(HCC)，Methods A total of 3023 individuats.including 1012 healthy controls.990 HCC-freeHBV-infected subjects。and l 02 1 HBV-caused hepatocellular carcinoma patients(HCC)were involvedin this study.PTPRD rs2279776 was genotypedusingquantitative PCR.HBV enhancer 1I/basal corepromoter/precore(Enh 1//BCP/preC)and preS regions were amplified by nested PCR and directlysequenced.Logistic regression analysis was performed to test the association among rs2279776polymorphism，HBV mutations，and their interactions on the risk of HCC.Results The distributionsof rs2279776 genotypes and allelic frequencies between HCC patients and healthy controlsHCCpatients and HBsAg.positive subjects without HCC。HCC patients and HCC-free population(HBsAg.positive suhjects without HCC and healthy controls)showed n0 statistically significant differences.However.the interactions of GC genotype on HBV mutations T1753V andpreSdeletionsignificantlyincreased on the risk of HCC in female HBV-infected subjects.Same result was also seen forrs2279776 C allele(GC+CC).The interaction ofrs2279776 GC genotype with G1896A could reducethe risk of HCC in H13V genotyve B infected subjects and the interaction of CC genotype withA1 652Gsignificantly reduced the risk of HCC in HBV genotype C infected subiects.ConclusionPTPRD rs2279776 didnotdirectlycontributetothegeneticsusceptibility on HCC risk.However.itmight affect the risk of HCC via interacting with HBV mutations.

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