| 刘建生,邵聪文,赵卫中,张云昆,吉玛,朱艳菊,马忠飞,马绍辉.2009年云南省柯萨奇病毒B5分离株A210／KM／09全基因序列分析[J].Chinese journal of Epidemiology,2014,35(3):307-311 |
| 2009年云南省柯萨奇病毒B5分离株A210／KM／09全基因序列分析 |
| Genomie characteristics of eoxsaclOevirIB5 A210／KM／09 strain isolated in Yunnan，China |
| Received:August 13, 2013 Revised:June 08, 2012 |
| DOI： |
| KeyWord: 柯萨奇B组5型病毒 全基因组 序列分析 进化树图 |
| English Key Word: Coxsaekievirus B5 Complete genome Sequence analysis Phylogenetie analysis |
| FundProject:云南省应用基础研究项目(2C13FZl36) |
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| Abstract: |
| 目的分析柯萨奇病毒B5(cvB5)云南分离株A210／KIVI／09全基因序列及其遗传特性。方法设计针对CVB5引物，提取病毒RNA，RT-PCR扩增和产物直接测序获得序列，利用Mega 4.1、RDP3和SimPlot 3.5.1软件分析全基因序列。结果CVB5 A210／K1w09全基因组核苷酸序列长度为7 372 bp，编码含2 18,5个氨基酸残基的多聚蛋白；A210／KNU09全基因组核苷酸及所推导的氨基酸与CVB5／CCl0／10同源性最高，其同源性分别为92.5%和97.3%；而与其他CVB5毒株如17Y、19CSF、20CSF、1954／85，US、2000／CSF／KOR、03001N、CoxB5／Henard2010、CVB5／SD／09和Faulkner核苷酸和氨基酸同源性分别为80.1%一925%和95.0%一97.3%；A210／K_M／09与其他CVB5毒株的各区段核苷酸和氨基酸同源性分别为75.3%～96.3%和85.3%，100.0%，其中与VP3、3D区段核苷酸的同源性最低。基于CVB5全长VPl基因序列进行种系进化分析，可将CVB5分为A、B、C和D4个基因型，其中C基因型可再分为CI～c4基因亚型，D基因型再分为D1。D4基因亚型。中国CVB5流行株主要聚集在C4基因亚型，国外流行株主要聚集在Dl、C2亚型。结论A2 10／KM／09分离株为C4：基因型。 |
| English Abstract: |
| Objective To characterize the complete genome sequence of coxsackievirus B5(CVB5) strainwhich was isolated fromYunnan，China，2009.Methods Eightoverlappingclones coveting the whole viral genome(excluding the poly-A tail)were obtained by ImPCR andsequenced.with their nucleotide and amino acid sequences compared with other known CVB5 strains.Results The genome of the CVB5 A210／KM／09 strain had 7 372 nucleotides in length，andcontaining a 742-nt non.translated region fNTR)at the 5 7 end and a 98-nt NTR at the 3’end.Theentire open reading frame contained 6 555 nt.encoding a 2 185-aa polyprotein.In the coding region.there appeared no nucleotide deletion or insertion，but some changes of amino acid seemed unique.Based on the complete genome sequence alignments，CVB5 isolatc A210／KMJ09 strain showed thehighest nucleotide(92.5%)and amino acid(97.3%)identities to the CVB5／CCl010.It also sharednucleotide(80.j%-92.5%)and amino acid(95.O%-97，3%)homology with other CVB5 strains：l 7Y，19CSF.20CSF，1 954／85／US，2000／CSF／KOR，03001N，CoxB5／Henan／20 1 0，VB5／SD／09 andFaulkner.Blast between genome fragments.A2lOⅨM，09 showed similarity onnucleotide(80.1%-92.5%)and amino acid(95.0%-97.3%)identities with other CVB5 strains.The phylogenetictree.constructed on the complete VP：L regions，indicated that CVB5 could be divided into genotype A，B.C and D.while Genotype C and D could be further divided into C1-C4 and D1-D4 subgenotypes.Conclusion A210／K／VI／09 and other CVB5 predominant strains isolated in China belonged to CVB5 subgenotype C4. |
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