| 汪永禄,李凤娟,王多春,张萍,陶勇,王利,王艳.马鞍山地区耐甲氧西林金黄色葡萄球菌的分子特征研究[J].Chinese journal of Epidemiology,2015,36(3):285-289 |
| 马鞍山地区耐甲氧西林金黄色葡萄球菌的分子特征研究 |
| Study of molecular characteristics of methicillin-resistant Staphylococcus aureus in Maanshan area |
| Received:September 10, 2014 |
| DOI：10.3760/cma.j.issn.0254-6450.2015.03.020 |
| KeyWord: 金黄色葡萄球菌 溶血素 多位点序列分型 药敏试验 |
| English Key Word: Staphylococcus aureus Hemolysin Multilocus sequence typing Drug sensitivity test |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wang Yonglu | Maanshan Municipal Center for Disease Control and Prevention (CDC), Maanshan 243000, China | mas_wyl@163.com | | Li Fengjuan | National Institute for Communicable Diseases Control and Prevention, Chinese CDC
CDC of Henan Province | | | Wang Duochun | National Institute for Communicable Diseases Control and Prevention, Chinese CDC | | | Zhang Ping | National Institute for Communicable Diseases Control and Prevention, Chinese CDC
CDC of Tongzhou District, Beijing | | | Tao Yong | Maanshan Municipal Center for Disease Control and Prevention (CDC), Maanshan 243000, China | | | Wang Li | Maanshan Municipal Center for Disease Control and Prevention (CDC), Maanshan 243000, China | | | Wang Yan | Maanshan Municipal Center for Disease Control and Prevention (CDC), Maanshan 243000, China | |
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| Abstract: |
| 目的 了解马鞍山地区耐甲氧西林金黄色葡萄球菌(金葡菌)(MRSA)肠毒素、溶血素分布情况、菌株克隆群关系及其耐药性。方法 采用全自动酶联荧光免疫系统和PCR技术分别检测肠毒素和溶血素基因分布;选择金葡菌的7个管家基因作为目的基因,对34株MRSA和3株甲氧西林敏感金葡菌(MSSA)进行多位点序列分型(MLST),然后与网上数据库比对,获得序列型(ST),根据eBURST的ST进行亲缘性分析;采用琼脂稀释法检测MRSA对12种抗生素的耐药情况。结果 210株金葡菌肠毒素阳性率为50.9%,溶血素基因携带率为97.1%,其中51株MRSA全部含有溶血素基因。34株MRSA有10个ST,以ST239为主(47.1%,16/34),其次为ST5(17.6%,6/34);3株MSSA的ST为ST188、ST1281和ST7。17株患者来源菌株分为6个ST,以ST239为主(35.3%,6/17),其次为ST5(29.4%,5/17);20株食品来源菌株有9个ST别,以ST239为主(45.0%,9/20),其次为ST7(15.0%,3/20)。ST585、ST630以及ST239的亲缘关系较近,其他ST之间亲缘关系较远。除万古霉素外,所有菌株对10种抗生素有不同程度的耐药。结论 金葡菌溶血素普遍存在;ST239为马鞍山地区MRSA的主要优势菌株,各ST间亲缘关系较远。 |
| English Abstract: |
| Objective To identity the distribution of enterotoxin and hemolysin,as well as the clonal complexes and drug resistance of the strains of methicillin-resistant Staphylococcus aureus (MRSA) in Maanshan region. Methods Automatic enzyme-linked fluorescent assay system and PCR technology were used to identify the distribution of enterotoxin and hemolysin genes. Seven Staphylococcus aureus hourskeeping genes were choosed as the target genes for multilocus sequence typing (MLST) on 34 strains of MRSA and 3 strains of methicillin-sensitive Staphylococcus aureus(MSSA),comparing the data with the online database and obtaining the sequence typing(ST),conducting affinity analysis on its ST based on eBURST,testing in agar dilution method the drug resistance of MRSA against 12 antibiotics. Results 50.9% of the 210 Staphylococcus aureus strains were enterotoxin positive,and 97.1% of them carried hemolysin genes as all 51 strains of MRSA carried hemolsin genes. The 34 MRSA strains were divided into 10 STs,ranging in sequence ST239 (47.1%,16/34),ST5 (17.6%,6/34). Three MSSA strains belonged to ST188,ST1281 and ST7,respectively. Seventeen strains from the patients were divided into 6 STs,ranging in sequence ST239 (35.3%,6/17) and ST5 (29.4%,5/17). Twenty strains from food sources were divided into 9 STs,ranging in sequence ST239 (45.0%,9/20) and ST7 (15.0%,3/20). STs of ST585,ST630 and ST239 were close in affinity,while the rest were distant in affinity. Except for vancomycin,all the strains were found with drug resistance to varying extent to the 10 antibiotics tested. Conclusion Existence of Staphylococcus aureus hemotoxin was universal;ST239 was the main predominant MRSA in Maanshan region,with distant affinity among the STs. |
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