| 杜小莉,李伟,阚飙,崔志刚,崔晶花.我国部分地区克罗诺杆菌分离株分种及其方法的比较研究[J].Chinese journal of Epidemiology,2016,37(2):259-262 |
| 我国部分地区克罗诺杆菌分离株分种及其方法的比较研究 |
| Study on identification of Cronobacter spp. species in 11 areas in China |
| Received:July 02, 2015 |
| DOI：10.3760/cma.j.issn.0254-6450.2016.02.022 |
| KeyWord: 克罗诺杆菌 序列分析 分种 |
| English Key Word: Cronobacter spp. Sequencing analysis Specie |
| FundProject:国家科技重大专项(2012ZX10004215; 2013ZX10004-101) |
| Author Name | Affiliation | E-mail | | Du Xiaoli | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Li Wei | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Kan Biao | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Cui Zhigang | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Cui Jinghua | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | cuijinghua@icdc.cn |
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| Abstract: |
| 目的 比较研究克罗诺杆菌分离株的分种方法,探讨其中准确、快速有效分种方法。方法 采用生化实验和对基因16S rRNA,fusA序列聚类分析的方法。结果 105株菌的4项生化结果呈现6种情况,但无法将C.turicensis和C.universalis两个种分开;基于16S rRNA基因分析分种结果将105株菌分为5组,无法将C.sakazakii和C.malonaticus这两个种的菌株有效地分开;而基于fusA基因分析分种结果可将所有菌株分为C.sakazakii(58株)、C.malonaticus(30株)、C.dublinensis(11株)、C.turicensis(5株)、C.muytjensii(1株)。结论 目前采用fusA序列分种相对简捷有效,但缺乏直观,需再研究达到快速检测目的的其他方法。 |
| English Abstract: |
| Objective To compare different Methods on the identification of Cronobacter(C.) spp.species and to choose an optimum one.Methods Biochemical test, 16S rRNA and fusA sequencing Methods were carried out.Results When using the biochemical test, 105 strains showed six different conditions but C.turicensis and C.universalis could not be effectively identified.Under the 16S rRNA sequencing analysis, all the strains were divided into 5 groups but C.sakazakii and C.malonaticus were tangled.Finally, all the strains were identified into 58 C.sakazakii, 30 C.malonaticus, 11 C.dublinensis, 5 C.turicensis, 1 C.muytjensii, under the fusA sequencing analysis.Conclusion Currently, fusA sequencing analysis seemed an effective method for identifying the species of Cronobacter.Since fusA sequencing analysis method was less intuitive, another method for rapid testing should be developed. |
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