| 李存香,王鹏,申小娜,张海鹏,丁奕博,陈邬锦,和映天,和琼光,黄英,李振军,李伟.野生型鼠疫噬菌体YP060的分离和生物学特性鉴定[J].Chinese journal of Epidemiology,2016,37(6):868-871 |
| 野生型鼠疫噬菌体YP060的分离和生物学特性鉴定 |
| Isolation and biological characteristics on Yersinia pestis phage YP060 |
| Received:March 15, 2016 |
| DOI：10.3760/cma.j.issn.0254-6450.2016.06.025 |
| KeyWord: 鼠疫 噬菌体 鼠疫菌 生物学特性 |
| English Key Word: Plague Phage Yersinia pestis Biological characteristics |
| FundProject: |
| Author Name | Affiliation | E-mail | | Li Cunxiang | State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Graduate School, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Department of Plague Control and Prevention, Qinghai Institute for Endemic Disease Control and Prevention, Xining 811602, China | | | Wang Peng | Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China | | | Shen Xiaona | State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Zhang Haipeng | Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China | | | Ding Yibo | Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China | | | Chen Wujin | Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China | | | He Yingtian | Department of Plague Control and Prevention, Yulong Center for Disease Control and Prevention, Lijiang 674100, China | | | He Qiongguang | Department of Plague Control and Prevention, Yulong Center for Disease Control and Prevention, Lijiang 674100, China | | | Huang Ying | State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | Li Zhenjun | Executive Office, Tibet Autonomous Region Center for Disease Control and Prevention, Lasa 850000, China | lizhenjun@icdc.cn | | Li Wei | State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | liwei@icdc.cn |
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| Abstract: |
| 目的 从鼠疫疫源地鼠巢中分离1株野生型鼠疫噬菌体(YP060)并分析其生物学特性。方法 采用双层琼脂平皿法从云南省鼠疫疫源地鼠巢中分离鼠疫噬菌体;描述其形态特征;了解裂解能力、宿主谱、最佳感染复数、一步生长特性;分析不同温度和pH值、紫外线、氯仿对噬菌体的敏感性。结果 YP060形态呈蝌蚪形,头部为正多面体结构,带有一伸缩的尾鞘;对鼠疫疫苗株EV76的最佳感染复数为0.1;潜伏期为50 min,暴发期为80 min;宿主谱评价显示,YP060仅裂解鼠疫疫苗株;YP060在30~50℃具有较强的热稳定性;pH值5~10范围内均有较强的裂解活性;对紫外线比较敏感;对氯仿不敏感,5%浓度的氯仿对其活性基本没有影响。结论 本研究为国内首次从鼠疫疫源地鼠巢中分离到鼠疫噬菌体YP060,该噬菌体具有窄宿主谱和较强的生物学裂解特性。 |
| English Abstract: |
| Objective To isolate and identify the characteristics of Yersinia pestis phage YP060 from mice nests in Yunnan plague focus. Methods Phage YP060 was isolated from Yunnan plague focus by double-layer agar plate method with attenuated vaccine Yersinia pestis strain EV76 as the host bacterium. Phage particle was observed under the electron microscope while biological properties of phage YP060 including lytic capacity, host spectrum, optimal multiplicity of infection (MOI), one-step growth curve, sensitivity to temperature, pH, ultraviolet ray and chloroform etc. were identified. Results The particles of phage YP060 presented tadpoles-like features, with optimal multiplicity of infection as 0.1. Latent period and burst period appeared as 50 min and 80 min, respectively. The phage endured the temperature between 30-50℃, and it contained a stronger lysis activities in a range of pH 5 to 10. Phage YP060 was sensitive to ultraviolet rays but insensitive to chloroform while presented special lysic ability to Yersinia pestis. Conclusion Yersinia pestis phage YP060 was isolated for the first time from mice nests in plague natural focus in China. Our findings related to narrow host spectrum and stronger lysis activities. |
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