| 孙扬,王友信,张杰,于鑫玮,盖思齐,王皓,董晶,郭秀花,王嵬.不同性别人群免疫球蛋白G N-糖基化结构谱型比较[J].Chinese journal of Epidemiology,2016,37(10):1409-1412 |
| 不同性别人群免疫球蛋白G N-糖基化结构谱型比较 |
| Comparison of gender specific structure profiles of immunoglobulin G N-glycans |
| Received:June 12, 2016 |
| DOI：10.3760/cma.j.issn.0254-6450.2016.10.018 |
| KeyWord: IgG N-糖基化 性别 |
| English Key Word: IgG N-glycosylation Gender |
| FundProject:国家自然科学基金（81530087，81373099） |
| Author Name | Affiliation | E-mail | | Sun Yang | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Wang Youxin | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Zhang Jie | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Yu Xinwei | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Ge Siqi | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Wang Hao | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | | | Dong Jing | Center for Physical Examination, Xuanwu Hospital, Capital Medical University, Beijing 100050, China | | | Guo Xiuhua | Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China | guoxiuh@ccmu.edu.cn | | Wang Wei | School of Medical Sciences and Health, Edith Cowan University, Joondalup WA6027, Australia | wei.wang@ecu.edu.un |
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| Abstract: |
| 目的 对不同性别人群的免疫球蛋白G（IgG） N-糖基结构谱型进行分析比较。方法 采用整群抽样，以2012年1-6月在北京市宣武医院体检的669名居民为研究对象，留取空腹静脉血5 ml，对IgG N-糖基进行分离、纯化、标记后，采用超高压液相色谱串联质谱法对IgG N-糖基进行检测，最终得到24个色谱峰，每个峰代表不同的糖基结构。不同性别人群的糖基结构比较采用两独立样本t检验或非参数检验，P<0.05为差异有统计学意义。结果 669名参与者中男性235人（35.13%），女性434人（64.87%），平均年龄为（44.23±9.82）岁。女性核心岩藻糖基化水平Fn（Z=-2.192，P=0.028）高于男性；女性半乳糖基化水平G0n（Z=-7.898，P<0.001）、G1n（Z=-2.343，P=0.019）低于男性，而G2n（Z=-8.414，P<0.001）水平高于男性；女性唾液酸化水平FtotalS1/FtotalS2（Z=-5.049，P<0.001）、FS1/FS2（Z=-3.336，P=0.001）高于男性。结论 IgG N-糖基结构水平可能与性别有关。 |
| English Abstract: |
| Objective To analyze and compare the gender specific structure profiles of immunoglobulin G(IgG) N-glycans and provide evidence to understand the distribution of the structure of IgG N-glycans in general population. Methods A cluster sampling was conducted to randomly select 669 participants from Beijing Xuanwu Hospital during January-June 2012. Venous blood sample (5 ml) was collected from each participant with vacuum negative pressure tube containing EDTA. Plasma IgG N-glycome was determined using ultra-performance liquid chromatography (UPLC) on Waters BEH Glycan chromatography column and mass spectrometry after the separation, purification and labeling. Finally, we got 24 chromatographic peaks. Each peak represented a different carbohydrate structure. Independent sample t-test or nonparametric test were used to compare the gender specific difference, P<0.05 was regarded as significant. Results A total of 669 healthy participants were included, including 235 men (35.13%) and 434 women (64.87%). The average age of the participants was (44.23±9.82) years. The core fucosylation level and the galactosylation level of females were higher than those of males[Fn(Z=-2.192, P=0.028), G0n(Z=-7.898, P<0.001), G1n(Z=-2.343, P=0.019), G2n(Z=-8.414, P<0.001)], but FtotalS1/FtotalS2(Z=-5.049, P<0.001) and FS1/FS2 (Z=-3.336, P=0.001) of females were higher than those of males, indicating a higher saliva acidification level in males than in females. Conclusion IgG N-glycosylation levels might be gender dependent. |
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