线粒体DNA拷贝数与相关因素的交互作用对煤矿工人高血压的影响

郭建勇; 雷立健; 乔楠; 范国权; 孙晨明; 黄建军; 王彤

Abstract

郭建勇,雷立健,乔楠,范国权,孙晨明,黄建军,王彤.线粒体DNA拷贝数与相关因素的交互作用对煤矿工人高血压的影响[J].Chinese journal of Epidemiology,2017,38(1):26-31

线粒体DNA拷贝数与相关因素的交互作用对煤矿工人高血压的影响

Research on potential interaction between mitochondrial DNA copy number and related factors on risk of hypertension in coal miners

Received:July 12, 2016

DOI：10.3760/cma.j.issn.0254-6450.2017.01.005

KeyWord: 线粒体高血压相关因素交互作用

English Key Word: Mitochodria Hypertension Related factors Interaction study

FundProject:山西省煤矿公共卫生研究生教育创新中心科研项目（KY2015006）；国家自然科学基金（81273040）

Author Name	Affiliation	E-mail
Guo Jianyong	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Lei Lijian	School of Public Health, Shanxi Medical University, Taiyuan 030001, China	wwdlijian@aliyun.com
Qiao Nan	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Fan Guoquan	School of Public Health, Shanxi Medical University, Taiyuan 030001, China
Sun Chenming	General Hospital of Datong Coal Mine Group, Datong 037000, China
Huang Jianjun	General Hospital of Datong Coal Mine Group, Datong 037000, China
Wang Tong	School of Public Health, Shanxi Medical University, Taiyuan 030001, China

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Abstract:

目的探讨煤矿工人线粒体DNA（mtDNA）拷贝数与相关因素对高血压的影响及其交互作用。方法采用病例对照研究设计，选取大同煤矿集团中378例患有高血压病的煤矿工人作为病例组，非高血压煤矿工人325例作为对照组。通过问卷调查收集研究对象基本情况、生活饮食习惯等信息，采用荧光定量PCR方法对外周血mtDNA进行扩增，定量分析mtDNA拷贝数的变异情况，采用logistic回归模型分析高血压的相关影响因素，并进行交互作用分析。结果相对于mtDNA拷贝数0～5.67组，具有较高水平的mtDNA拷贝数者高血压患病风险较低，但差异无统计学意义（P=0.414）。饮酒（OR=1.80，95% CI：1.26～2.56）、高血压家族史（OR=1.74，95% CI：1.20～2.50）、倒班（OR=0.69，95% CI：0.48～0.99）、文化程度（P=0.012）和家庭收入（P<0.001）是高血压患病的相关因素。构建mtDNA拷贝数与相关因素的交互作用项，分析结果显示，饮酒与mtDNA拷贝数、高血压家族史与mtDNA拷贝数、家庭收入与mtDNA拷贝数存在相乘的交互作用。饮酒是高血压患病的危险因素［1.77（1.25～2.50）］，mtDNA高拷贝数与其交互作用使高血压患病风险降低（交互作用OR=1.20，95% CI：1.07～1.35）；高血压家族史是患高血压病的危险因素［1.81（1.26～2.59）］，mtDNA高拷贝数与其交互作用使患高血压患病风险降低（交互作用OR=1.24，95% CI：1.09～1.41）；家庭收入是高血压的保护因素［0.55（0.46～0.66）］，mtDNA高拷贝数与其交互作用使其对高血压患病的保护作用减弱（交互作用OR=0.90，95% CI：0.86～0.94）。结论不同mtDNA拷贝数的研究对象高血压患病风险无统计学意义，但mtDNA高拷贝数与饮酒、家庭收入、高血压家族史之间的相乘交互作用使后三者对高血压的影响降低。

English Abstract:

Objective To investigate the effects of mitochondrial DNA (mtDNA) copy number in peripheral blood and related factors on the risk of hypertension in coal miners. Methods A case-control study was conducted in 378 coal miners with hypertension and 325 healthy coal miners recruited from Datong Coal Mine Group. A standard questionnaire was used to collect their general information, such as demographic characteristics, habits and occupational history. Fluorescence quantitative PCR was performed to detect the copy number of mtDNA. Logistic regression model was applied for identifying the related risk factors of hypertension and analyzing the interaction between mtDNA copy number and risk factors. Results The prevalence of hypertension of high mtDNA copy number was lower than mtDNA copy numberin 0-5.67 group, but the difference was not statistically significant (P=0.414). Alcohol drinking (OR=1.80, 95%CI:1.26-2.56), family history of hypertension (OR=1.74, 95%CI:1.20-2.50), work shifts (OR=0.69, 95%CI:0.48-0.99), education level (P=0.012) and family monthly income level (P=0.001) were related to the prevalence of hypertension. There were potential interactions between mtDNA copy number and alcohol drinking, family monthly income level, family history of hypertension, respectively. Alcohol drinking was a risk factor for hypertension[1.77(1.25-2.50)]. Potential interactions between mtDNA copy number and alcohol drinking reduced the risk of hypertension (OR=1.20, 95%CI:1.07-1.35). Family history of hypertension was a risk factor for hypertension[1.81(1.26-2.59)]. Potential interactions between mtDNA copy number and family history of hypertension reduced the risk of hypertension (OR=1.24, 95%CI:1.09-1.41). Family monthly income level was a protect factor for hypertension[0.55(0.46-0.66)]. Potential interactions between mtDNA copy number and family monthly income level increased the protection role of hypertension (OR=0.90, 95%CI:0.86-0.94). Conclusion mtDNA copy number variation was not significantly associated with the prevalence of hypertension in coal miners, but mtDNA copy number showed multiplication interaction on the prevalence of hypertension with alcohol drinking, family monthly income level as well as family history of hypertension and made their influences weaken.

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